Flowcytometric analysis

Spermatogonial Stem Cells (SSCs) are unipotent adult stem cells, defined as the undifferentiated stem cells located along the basement membrane of the seminiferous tubules. This present study is aimed to carry out the culture and characterization of SSCs in pre-pubertal age group of mice. Isolation of SSCs was done by two-step enzymatic digestion method and was seeded in a six-well plate. In primary culture (P0), colony forming unit was observed on day four post incubation. Mice Spermatogonial Stem Cells (mSSCs) adherence was observed on day eight post incubation.

7p nguaconbaynhay6 24-06-2020 19 0   Download

Bursa of Fabricius, the primary lymphoid organ for B-lymphopoiesis in Avian species and is responsible for humoral immunity. Flow cytometric analysis of Bu-1a+ B-cell count and CD3+, CD4+ and CD8+ T-cell count were conducted in bursa of Fabricius of four different age groups of Nandanam broiler chicken viz., day-old, two, four and six weeks. Twelve birds from each age group, from both vaccinated and non-vaccinated groups were utilised. Tissue samples of the bursa were collected aseptically and processed for flow cytometric studies using monoclonal antibodies.

6p nguaconbaynhay6 24-06-2020 10 0   Download

Flowcytometric analysis of T lymphocyte subset viz., CD and CD8 cells in spleen and thymus was done in Nandanam layer chicken of different age groups ranging from day-old to forty weeks. . In spleen, the mean CD4 and CD8 population was found to be the highest at day one and lowest at twenty weeks of age. However, an increase in both the T cell population was detected at forty weeks.

5p nguaconbaynhay6 23-06-2020 9 0   Download

Earlier we presented several lines of evidence that a 67-kDa laminin binding protein (LBP) inLeishmania donovani,that is different from the putative mammalian 67-kDa laminin receptor, may play an important role in the onset of leish-maniasis, as these parasites invade macrophages in various organs after migrating through the extracellular matrix. Here we describe the membrane orientation of thisLeish-manialaminin receptor.

8p tumor12 20-04-2013 42 2   Download