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Fungal DNA and amplified
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We present a case of sporotrichosis diagnosed by molecular biology combined with traditional methods. Fungal DNA was extracted from skin tissues of the patient by fungal DNA extraction kit. The PCR amplified fragments were compared on GenBank website, and the pathogenic fungi were identified.
4p
viintuit
26-09-2023
1
0
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The present study was taken up in the Agricultural College, Bapatla during 2016-2017. Cercospora infected leaves were collected during kharif 2016 from eight different okra growing villages in Guntur district, Andhra Pradesh which were used for in planta isolation of fungal DNA and amplified using universal primers ITS 1 and ITS 4. The 550 bp amplicon thus obtained was restricted with hexa cutters, EcoRI, BamHI and tetra cutter Taq1 to find variability among Cercospora isolates.
7p
gaocaolon5
14-06-2020
8
1
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The fungus Venturia carpophila causes scab in almond. In order to gain insight, an in vitro culture of the fungus has been established and its identity confirmed by its nr DNA. Internal transcribed spacer-ribosomal DNA (ITS-rDNA); the fungal molecular marker was used for molecular analysis. The target region of rDNA (ITS1-5.8S-ITS2) of this species was amplified using universal fungal primers (ITS1 and ITS4).
7p
chauchaungayxua3
07-02-2020
12
1
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A gene encoding β-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia coli. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16.
10p
vivant2711
01-02-2019
20
2
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Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice’s coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates.
10p
masterbarista
15-01-2019
15
1
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