Fusarium wilt and molecular analysis

Banana Fusarium wilt is a devastating disease of bananas caused by Fusarium oxysporum f. sp. cubense (Foc) and is a serious threat to the global banana industry. Knowledge of the pathogenic molecular mechanism and interaction between the host and Foc is limited.

17p vihagrid 30-01-2023 9 3   Download

The fungal pathogen Fusarium oxysporum f.sp. pisi (Fop) causes Fusarium wilt in peas. There are four races globally: 1, 2, 5 and 6 and all of these races are present in Australia. Molecular infection mechanisms have been studied in a few other F. oxysporum formae speciales; However, there has been no transcriptomic Fop-pea pathosystem study.

18p vilarryellison 29-10-2021 7 0   Download

Development and application of DNA-based methods to distinguish highly virulent isolates of Fusarium oxysporum f. sp. koae [Fo koae; cause of koa wilt disease on Acacia koa (koa)] will help disease management through early detection, enhanced monitoring, and improved disease resistance-breeding programs.

15p vijeeni2711 30-06-2021 10 1   Download

The study was undertaken to isolate the DNA of good quality from the banana wilt pathogen Fusarium oxysporum f. sp. cubense (Foc) directly from infected tissues for molecular studies. In this method, laborious and time-consuming procedures such as isolation of the pathogen and its artificial cultivation are avoided. Moreover, grinding of the fungal mycelium using liquid nitrogen is also not needed as the DNA is directly extracting from infected banana tissues.

7p nguaconbaynhay7 15-08-2020 13 1   Download

Fusarium oxysporum f. sp. cumini is a serious disease of cumin (Cuminum cyminum L.). Ten isolates of F. oxysporum f. sp. cumini were isolated from major cumin growing districts of Gujarat state to compare molecular methods over traditional for differentiation among the isolates of pathogen. The average 98.70 % of polymorphism was recorded for all the 11 ISSR primers. The polymorphic information content (PIC) value ranged from 0.571 to 0.831 with an average of 0.739 per primer. The IISR primers grouped majority of the isolates based on their geographical location.

9p kethamoi6 29-06-2020 19 0   Download

Banana and plantains is the most important tropical fruit crop in the world, which serves as a major food and source of income in many countries. Bananas are severely infected by several pathogens one such Fusarium oxysoporum f. sp. Cubense which causes significant crop losses in the fields. In this study fusarium wilt screening was carried out on chemically induced mutants and molecular analysis was done to assess the acquired resistance to Fusarium oxysoporum f. sp. cubense.

9p trinhthamhodang1212 06-04-2020 10 1   Download

Regionally grown banana cultivars are severely infected by several pathogens one such Fusarium oxysoporum f. sp. Cubense which causes significant crop losses in the fields. In this study fusarium wilt screening was carried out on gamma ray induced mutants and molecular characterization was done to assess the acquired resistance to Fusarium oxysoporum f. sp. cubense. From the screening we observed that gamma ray derived mutants are more sensitive in 25, 30 and 35 Gy. Whereas, several mutants obtained from 40 and 45 were resistant as comparable with untreated control.

13p kequaidan2 13-12-2019 20 0   Download

The molecular evaluation of twelve chickpea (Cicer arietinum L.) varieties (screened for their resistance to fusarium wilt) at the Department of Plant Pathology and Agriculture Microbiology in 2014-15 was conducted to assess the genetic diversity and relationship of chickpea genotypes using RAPD. Twenty five primers of RAPD were used of which 15 primers gave amplification products. A total 349 amplicons were obtained of which 331 amplicons were polymorphic with 93.64 % level of polymorphism was observed.

9p cothumenhmong1 08-12-2019 16 0   Download