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Gene clone
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The study was carried out to clone sheep cytokine genes for protein expression in mammalian cell culture. Once expressed, it is hypothesized that these proteins, when added to a primary mast cell culture, will allow the growth of these cells.
15p
viambani
18-06-2024
2
0
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Glutamine synthetase (EC 6.3.1.2) is an enzyme which assimilates ammonium into amino acids. Therefore, GS plays an essential role in the metabolism of nitrogen. In vascular plants, GS is devided into two groups: cytosol - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 transgenic plants have been reported in many researches.
7p
vilarry
01-04-2024
6
1
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The objective of this study is to successfully clone and express P46 of M. hyopneumoniae in Escherichia coli M15 cells to create an initial material source for developing effective solutions for the prevention and treatment of the PEP caused by M. hyopneumoniae bacteria.
13p
viritesh
02-04-2024
3
1
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Recent sequencing studies on healthy skin and esophagus have found that, as we age, these tissues become colonized by mutant clones of cells carrying driver mutations in traditional cancer genes. This comment summarizes these findings and discusses their possible implications for our understanding of cancer, ageing, and other diseases.
3p
vibransone
28-03-2024
4
2
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Ebook "Phenolic compound biochemistry" is written for researchers, instructors, advanced undergraduate students and beginning graduate students in the life sciences who wish to become more familiar with these and many other intriguing aspects of phenolic compounds. Topics covered include nomenclature, chemical properties, biosynthesis, including an up-to-date overview of the genetics controlling phenolic metabolism, isolation and characterization of phenolic compounds, phenolics used in plant defense, and the impact of phenolics on human health.
284p
tachieuhoa
28-01-2024
6
2
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Part 1 book "Rabbit biotechnology - Rabbit genomics, transgenesis, cloning and models" includes content: Improvement of rabbit production, basic methods for experimental rabbits, useful information for rabbit genes, proteins, and antibodies, general physiology of rabbits, rabbit transgenesis, rabbit as a model for the study of human diseases.
72p
oursky05
20-09-2023
2
1
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Part 1 book "RT-PCR Protocols" includes content: Single cell RT-PCR on mouse embryos - A general approach for developmental biology; detection of influenza a virus neuraminidase and pb2 gene segments by one step reverse transcription polymerase chain reaction; RT-PCR amplification and cloning of large viral sequences,... and other contens.
189p
oursky05
20-09-2023
1
0
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Part 1 book "Animal cell technology, from biopharmaceuticals to gene therapy" includes content: Introduction to animal cell technology, animal cells - basic concepts, cloning and expression of heterologous proteins in animal cells, cell metabolism and its control in culture, culture media for animal cells, post-translational modification of recombinant proteins, mechanisms of cell proliferation and cell death in animal cell culture in vitro, mathematical models for growth and product synthesis in animal cell culture.
259p
oursky05
20-09-2023
7
3
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Aeromonas spp. uses T3SS to secrete and transport effector proteins to the host cells. These proteins play a major role in bacteria virulence by interfering with the signaling cascades and disrupting the cytoskeleton structure of the host cell.
6p
vicaptainmarvel
21-04-2023
8
2
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Prodigiosin (Pg), a secondary metabolite with anticancer and antimicrobial activities, can be produced in Serratia marcescens bacteria through the condensation reaction of 4-methoxy-2, 2’- bipyrrole-5-carboxyaldehyde (MBC) and 2-methyl-3-amylpyrrole (MAP).
9p
vicaptainmarvel
21-04-2023
6
2
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The poultry industry in Vietnam is vulnerable to many viral diseases. Since antibiotics and vaccination provide inadequate protection, it will be beneficial to have alternative immunostimulants to boost non-specific immunity in poultry. Chicken interferon-alpha (ChIFNα) has been described previously with antiviral activity against many pathogens.
9p
vicaptainmarvel
21-04-2023
4
2
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Dopamine β-hydroxylase (DBH) is a critical enzyme in the biosynthesis of catecholamines. This enzyme’s role in neuroendocrine regulation is well known, but there are some indications that it may also modulate reproduction and endocrine in mammals and birds.
9p
vinarcissa
21-03-2023
2
1
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Most agronomical traits of crop species are complex traits controlled by multiple genes and affected by environmental factors. While considerable efforts have been made to fine-map and clone major quantitative trait loci (QTLs) for yield-related traits in rice, it is not until recently that the attention has been paid to minor QTLs.
10p
vinarcissa
21-03-2023
7
1
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High-resolution mapping of the loci (QTN) responsible for genetic variation in quantitative traits is essential for positional cloning of candidate genes, and for effective marker assisted selection. The confidence interval (QTL) flanking the point estimate of QTN-location is proportional to the number of individuals in the mapping population carrying chromosomes recombinant in the given interval.
16p
vinarcissa
21-03-2023
2
1
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Grain zinc and iron concentration is a complex trait that is controlled by quantitative trait loci (QTL) and is important for maintaining body health. Despite the substantial effort that has been put into identifying QTL for grain zinc and iron concentration, the integration of independent QTL is useful for understanding the genetic foundation of traits.
15p
vinarcissa
21-03-2023
4
1
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Rosaceae is a family containing many economically important fruit and ornamental species. Although fluorescence in situ hybridization (FISH)-based physical mapping of plant genomes is a valuable tool for map-based cloning, comparative genomics and evolutionary studies, no studies using high resolution physical mapping have been performed in this family.
10p
vinarcissa
21-03-2023
2
1
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This study aimed to examine the effect of HDACi treatment during the ZGA period. In this study, the effect of HDACi treatment with different concentrations on preimplantation development of cloned bovine embryos is done.
7p
vimalfoy
08-02-2023
6
1
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Production of cloned bovine has many practical applications in medicine and agriculture, although low efficiency has still been the main problem. This challenge has resulted from improper epigenetic reprogramming of donor's nuclear such as histone acetylation and zygotic gene activation that does not occur correctly.
8p
vimalfoy
08-02-2023
3
1
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In this study, alkyl hydroperoxide reductase (AhpC) from M. bovis was evaluated as a new candidate vaccine antigen against TB in BALB/c mice model. The ahpC gene was amplified from M.bovis genome, cloned, and expressed in Escherichia coli. Vaccine antigen AhpC was formulated with Montanide ISA 61 VG, an oil-based emulsion adjuvant. Both IgG and IL-12 responses were observed in mice after administering the formulation both as a subunit vaccine alone and also as a booster vaccine for BCG immunization.
14p
lyhuyenthu
31-01-2023
6
2
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GATA1 is a key transcription factor in the GATA family, and promotes the differentiation and maturation of red blood cell, which is essential for normal hematopoiesis. Our study provides cues for further studying the roles of GATA1 gene in fish development, and suggested a potential molecular mechanism by which NP leads to abnormal development of fish embryos.
13p
vihagrid
30-01-2023
7
3
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