Gene silencing

In this study, the Mgra16281 gene (ID: MK322955.1) enco ding an effector with the unknown function was cloned from the rice ro ot-knot nematode Meloidogyne graminicola isolated in Long An province. To knock-down the expression of this gene, an artificial microRNA was synthesized based on the Osa-MIR528 precursor and inserted into an expression vector.

9p larachdumlanat129 19-01-2021 15 2   Download

The development of fruits without fertilization is known as parthenocarpy. Parthenocarpy improves the fruit quality, processing attributes, production and productivity of vegetable crops like tomato, cucumber, watermelon etc. Absence of seeds can enhances the shelf life of the fruits, allowing a better conservation, fruit set in adverse climatic conditions, early and offseason production of vegetable crops. Therefore, it is important to ensure yield stability regardless of environmental conditions.

20p gaocaolon5 14-06-2020 16 3   Download

High-molecular-weight glutenin subunits (HMW-GS) play important roles in the elasticity of dough made from wheat. The HMW-GS null line is useful for studying the contribution of HMW-GS to the end-use quality of wheat.

12p vishikamaru2711 29-04-2020 15 1   Download

The Sp family is a family of transcription factors that bind to cis-elements in the promoter regions of various genes. Regulation of transcription by Sp proteins is based on interactions between a GC-rich binding site (GGGCGG) in DNA and C-terminal zinc finger motifs in the proteins. In this study, we characterized the GC-rich promoter of the gene for the DNA methyltransferase (Dnmt1) that is responsible for methylation of cytosine residues in mammals and plays a role in gene silencing.

10p system191 01-06-2013 38 4   Download

Synaptophysin, one of the major proteins on synaptic vesi-cles, is ubiquitously expressed throughout the brain. Syn-aptophysin and synapsin I, another synaptic vesicle protein, are also expressed by retinoic acid-induced neuronally dif-ferentiated P19 teratocarcinoma cells. Here, we show that inhibition of histone deacetylase activity in P19 cells is suf-ficient to activate transcription of the synaptophysin and synapsin I genes, indicating that neuronal differentiation and impairment of histone deacetylases results in a similar gene expression pattern....

8p tumor12 20-04-2013 29 1   Download

Although ubiquitously expressed, the transcriptional factor CP2 also exhib-its some tissue- or stage-specific activation toward certain genes such as globin in red blood cells and interleukin-4 in T helper cells. Because this specificity may be achieved by interaction with other proteins, we screened a peptide display library and identified four consensus motifs in numerous CP2-binding peptides: HXPR, PHL, ASR and PXHXH. Protein-database searching revealed that RE-1 silencing factor (REST), Yin-Yang1 (YY1) and five other proteins have one or two of these CP2-binding motifs....

13p awards 06-04-2013 38 2   Download

Lack of functional Fragile X mental retardation protein (FMRP) is the pri-mary cause of the Fragile-mental retardation syndrome in humans. In most cases, the disease results from transcriptional silencing of fragile mental retardation gene 1, fmr1, which encodes FMRP. However, a single mis-sense mutation (I304N) in the second KH domain of FMRP gives rise to a particularly severe case of Fragile X syndrome.

0p awards 05-04-2013 37 1   Download

The human adenine nucleotide translocase-2 (ANT2) pro-moter contains a silencer region that confers partial repres-sion on the heterologous herpes simplex virus thymidine kinase (HSVtk) promoter [Barath, P., Albert-Fournier, B., Luciakova, K., Nelson, B.D. (1999)J. Biol. Chem.274, 3378–3384]. Two sequences in the silencer (Site-2 and Site-3) are protected in the DNase I assayin vitro, and one of these is a repeated GTCCTG element previously shown to act as theactive repressor element.

8p dell39 03-04-2013 41 3   Download

The bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phospho-gluconolactonase (G6PD-6PGL) found in Plasmodium falciparum has unique structural and functional characteristics restricted to this genus. This study was designed to examine the effects of RNA-mediated PfG6PD-6PGL gene silencing in cultures ofP. falciparumon the expression of para-site antioxidant defense genes at the transcription level.

10p inspiron33 26-03-2013 48 5   Download

RNA interference (RNAi) mediates sequence-specific post-transcriptional gene silencing in many eukaryotes and is used for reverse genetic studies and therapeutics. RNAi is triggered by double-stranded small interfering RNAs (siRNAs), which can be processed from small hairpin RNAs gener-ated from an expression vector.

7p inspiron33 23-03-2013 37 2   Download

The nuclear envelope (NE) is composed of inner and outer nuclear mem-branes (INM and ONM, respectively), nuclear pore complexes and an underlying mesh like supportive structure – the lamina. It has long been known that heterochromatin clusters at the nuclear periphery adjacent to the nuclear lamina, hinting that proteins of the lamina may participate in regulation of gene expression.

10p galaxyss3 21-03-2013 39 3   Download

RNA-mediated gene silencing (RNA interference) is a powerful way to knock down gene expression and has revolutionized the fields of cellular and molecular biology. Indeed, the transfection of cultured cells with small interfering RNAs (siRNAs) is currently considered to be the best and easi-est approach to loss-of-function experiments.

16p media19 06-03-2013 47 5   Download

The putative RNA-binding protein SUPPRESSOR OF GENE SILEN-CING 3 (SGS3) protects RNA from degradation before transformation into dsRNA by the RNA-dependent RNA polymerase RDR6 during plant post-transcriptional gene silencing and trans-acting small interfering (siRNA) pathways.

10p vinaphone15 27-02-2013 24 1   Download

To elucidate the function of kappa class glutathione transferases (GSTs) in multicellular organisms, their expression and silencing were investigated in Caenorhabditis elegans. In contrast with most vertebrates, which possess only one GST kappagene, two distinct genes encoding GSTK-1 and GSTK-2 are present in theC. elegansgenome.

11p vinaphone15 25-02-2013 36 2   Download

Như chúng ta đã biết trong tế bào có nhiều RNA khác nhau, mỗi loại đảm nhận một chức năng sinh học riêng biệt. Trong đó siARN thường được tạo ra từ các phân tử ARN sợi kép. RNA silencing là một thuật ngữ dùng để chỉ một hiện tượng gọi là " sự câm lặng” của RNA. Kể từ khi khám phá ra siRNA việc nghiên cứu cơ chế và ứng dụng RNA silencing được các nhà khoa học xem là "bước đột phá” trong ngành sinh học phân tử. ...

36p trautuongquan 25-02-2013 170 25   Download

This study investigated the potential of RNA interference, which is techni-cally challenging in bivalve mollusc species, to assess gene function in the oysterCrassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivoinjection of oyvl-dsRNA into the gonad provokes a knockdown phenotype correspond-ing to germ cell underproliferation and prematurely arrested meiosis throu-gout the organ.

8p viettel02 22-02-2013 32 2   Download

RNA silencing is an epigenetic inhibition of gene expression and is guided by small interfering RNAs. Sense transgene-induced post-transcriptional gene silencing (S-PTGS) occurs in a portion of a transgenic plant popula-tion. When a sense transgene encoding a tobacco endoplasmic reticulum x-3 fatty acid desaturase (NtFAD3) was introduced into tobacco plants, an S-PTGS line, S44, was obtained.

9p mobifone23 18-01-2013 45 3   Download

Death-associated protein kinase (DAPK) is a pro-apoptotic serine⁄threo-nine protein kinase that is dysregulated in a wide variety of cancers. The mechanism by which this occurs has largely been attributed to promoter hypermethylation, which results in gene silencing.

7p mobifone23 18-01-2013 42 5   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4

8p dauphong4 03-01-2012 62 8   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Selective gene silencing by viral delivery of short hairpin RNA

11p dauphong2 23-12-2011 40 5   Download