General transcription factors

Nuclear receptors (NRs) are ligand-regulated, DNA-binding transcription factors that function in the chromatin environment of the nucleus to alter the expression of subsets of hormone-responsive genes. It is clear that chromatin, rather than being a passive player, has a profound effect on both transcriptional repression and activation mediated by NRs.

9p research12 01-06-2013 32 2   Download

Minichromosomemaintenance (MCM) proteins are part of the replication licensing factor (RLF-M), which limits the initiationofDNAreplication to once per cell cycle.We have previously reported that higher order complexes of mam-malian pol II and general pol II transcription factors, referred to as pol II holoenzyme, also contain MCM pro-teins. In the present study we have analyzed in detail the interaction between MCM2 and pol II holoenzyme. N- and C- terminal deletions were introduced into epitope-tagged MCM2 and the truncated proteins were transiently expressed in 293 cells. ...

11p tumor12 22-04-2013 39 3   Download

RNA polymerase II (RNAPII) requires a set of general transcription factors )TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH)to initiate transcription from a gene promoterin vitro. General transcription factors have been isolated from Saccharomyces cerevisiae,rat, human and Drosophila,and their corresponding cDNAs have been cloned. In this report, we describe a reconstitutedin vitro transcription system that consists of the following prepara-tions of factors from the yeastSchizosaccharomyces pombe: affinity-purified RNAPII, TFIIH, and recombinant TBP, TFIIB, TFIIE and TFIIF. ...

12p dell39 03-04-2013 62 3   Download

As a critical step of the preinitiation complex assembly in transcription, the general transcription factor TFIIB forms a complex with the TATA-box binding protein (TBP) bound to a promoter element. Transcriptional activators such as the herpes simplex virus VP16 facilitate this com-plex formation through conformational activation of TFIIB, a focal molecule of transcriptional initiation and activation.

9p dell39 03-04-2013 33 2   Download

To elucidate the molecular mechanism underlying the heat shock response in cold-water fish species, genes enco-ding heat shock transcription factors (HSFs) were cloned fromRTG-2cellsof the rainbowtroutOncorhynchusmykiss. Consequently, two distinctHSF1genes, namedHSF1aand HSF1b, were identified. The predicted amino acid sequence ofHSF1ashows 86.4% identity to that ofHSF1b.Thetwo proteins contained the general structuralmotifs ofHSF1, i.e. a DNA-binding domain, hydrophobic heptad repeats and nuclear localization signals. ...

10p dell39 03-04-2013 42 4   Download

Human papillomavirus type 16 (HPV16) DNA replication, which requires two viral proteins E1 and E2, occurs only in the differentiating epithelium. Besides the general factors necessary for cellular DNA synthesis, other unidentified cellular factors are assumed to be involved in the regulation of HPV DNA replication.

13p galaxyss3 07-03-2013 42 2   Download

We report here on the formation of a complex between the two NrpR homologs present inMethanosarcina mazeiGo¨1 and their binding proper-ties to the nifHandglnK1 promoters. Reciprocal co-chromatography dem-onstrated that NrpRI forms stable complexes with NrpRII (at an NrpRI : NrpRII molar ratio of1 : 3), which are not affected by 2-oxo-glutarate.

14p viettel02 19-02-2013 28 3   Download

TnrA is a master transcription factor regulating nitrogen metabolism in Bacillus subtilisunder conditions of nitrogen limitation. When the preferred nitrogen source is in excess, feedback-inhibited glutamine synthetase (GS) has been shown to bind TnrA and disable its activity.

11p cosis54 05-01-2013 34 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Ossification process involving the human thoracic ligamentum flavum: role of transcription factors...

34p thulanh16 22-10-2011 40 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients...

9p thulanh13 15-10-2011 54 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The utility of pathway selective estrogen receptor ligands that inhibit nuclear factor-κB transcriptional activity in models of rheumatoid arthritis...

12p thulanh12 13-10-2011 44 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Transcriptional regulation of collagenase (MMP-1, MMP-13) genes in arthritis: integration of complex signaling pathways for the recruitment of gene-specific transcription factors...

8p thulanh12 12-10-2011 56 4   Download