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Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1
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To elucidate the molecular mechanism underlying the heat shock response in cold-water fish species, genes enco-ding heat shock transcription factors (HSFs) were cloned fromRTG-2cellsof the rainbowtroutOncorhynchusmykiss. Consequently, two distinctHSF1genes, namedHSF1aand HSF1b, were identified. The predicted amino acid sequence ofHSF1ashows 86.4% identity to that ofHSF1b.Thetwo proteins contained the general structuralmotifs ofHSF1, i.e. a DNA-binding domain, hydrophobic heptad repeats and nuclear localization signals. ...
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