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Generation stable cell line used
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Transposon-mediated, non-viral gene delivery is a powerful tool for generating stable cell lines and transgenic animals. However, as multi-copy insertion is the preferred integration pattern, there is the potential for uncontrolled changes in endogenous gene expression and detrimental effects in cells or animals. Our group has previously reported on the generation of several transgenic cattle by using microinjection of the Sleeping Beauty (SB) and PiggyBac (PB) transposons and seeks to explore the long-term effects of this technology on cattle.
12p
vibeauty
23-10-2021
7
0
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The ecdysone inducible system was generated for checking the the toxicity of the target gene which was transfected into the cells. The retinoid X receptor (RXR) was transfected into HEK293FT to generate an inducible stable cell line pVgRXR-HEK293FT. For checking the function, the cells transfected with pIND-GFP and induced by 1µM ponasteron A for GFP expression. The fluorescence signals were detected in the transfected and induced cells informed the success of experiment.
7p
trinhthamhodang1218
18-03-2021
11
1
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Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use.
15p
vihamax2711
21-04-2020
17
2
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