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Genetic transformation protocol
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Ebook "Genetic transformation systems in fungi (Volume 1)" will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide.
310p
ladongphongthanh1008
22-04-2024
2
2
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A protocol of the Agrobacterium - mediated transformation of Eucalyptus urophylla cotyledon was successfully developed. This study aims to establish the procedure for transformation and regeneration of Eucalyptus urophylla cotyledon using Agrobacterium tumefaciens.
8p
vilarry
01-04-2024
7
0
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Ebook "Proteases and cancer: Methods and protocols" explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography, angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases.
355p
lucchinguyen
28-12-2023
3
2
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In order to set up a system for production of recombinant proteins using suspension cell culture of Nicotiana tabacum cv. Bright Yellow 2 (BY-2), in vitro culture and genetic transformation protocols of such cells were optimised at the laboratory of Plant Cell Biotechnology, Institute of Biotechnology.
10p
tamynhan8
04-11-2020
8
1
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The purpose of this study was to develop an efficient protocol for genotype independent gene transformation in cotton (Gossypium hirusutum) a worldwide commercially important fibre crop, to reduce the adverse impact of harmful chemicals used to control biotic stress. Most cotton varieties remain recalcitrant and amenable to genetic manipulation to protocols so far developed.
13p
trinhthamhodang7
31-08-2020
12
1
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Aromatic rice is profitable farming venture and having high consumer demand. The main concerns about aromatic rice are low yield and susceptibility to pests and diseases. A rapid and reproducible genetic transformation protocol was optimized for three aromatic rice varieties using the established plant regeneration protocol.
30p
nguaconbaynhay6
23-06-2020
11
1
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In vitro regeneration and in planta transformation protocols were standardized for the black gram (Vigna mungo L.) cv. VBN 3, an important grain legume. An efficient regeneration protocol was developed using 1-week-old de-embryonated cotyledon as explants. The maximum frequency of shoot regeneration (72.6%), multiple shoot induction (6.0 ± 0.3) and shoot elongation was achieved on MS medium supplemented with 3.0 mg/l 6-benzyl amino purine (BAP) after 4 weeks of culture. Up to 65% of the regenerated shoots were rooted on MS medium containing 0.
15p
angicungduoc4
26-04-2020
17
0
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The biotechnological intervention to enhance crop productivity via genetic transformation mainly depends upon an efficient regeneration protocol. Japonica, Indica and Basmati varieties of rice vary highly in their regeneration capabilities. Establishment of a highly efficient regeneration system for mature embryos in rice will enable transformation of foreign genes in rice.
7p
nguaconbaynhay4
22-03-2020
11
2
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Taif is one of the oldest cities in the world. It is located in the western part of Saudi Arabia, Taif rose is considered as one of the most important economic products of Taif governorate, KSA. The major use of Damask rose is distillation of petals in order to extract its essential oil and producing rosewater. Due to the heterozygsity, high degree of sterility and difficulty of breeding programs in Roses, therefore, the genetic transfer is the appropriate way to increase productivity and improve quality.
9p
bichxuan01643027348
21-10-2019
27
0
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Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement. The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz.
7p
sony2711
01-02-2019
13
2
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Solanum trilobatum L. is one of the most valued medicinal plants of Indian Ayurveda and Siddha medicine. An efficient protocol for direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation using in vitro derived leaf explants of S. trilobatum was established for the first time.
12p
sony2711
28-01-2019
34
2
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Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies.
7p
vimb123
11-01-2019
28
2
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