Genetically engineered proteins

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Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry

Division of Medicinal and Natural Products Chemistry, Department of Chemistry and Department of Chemical Engineering, University of Iowa, USA; 2Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark Synthetic peptides based on amino-acid residues 27–38 of human serum amyloid P component represent a novel type of heparin binders as they do not contain clusters of basic amino acids or other known features associated with protein or peptide heparin binding.

8p system191 01-06-2013 54 4 Download

Báo cáo khoa học: Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64

The envelope protein gp64 of the baculovirusAutographa californicanuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein bothmediates pH-dependentmembrane fusionandbinds tohost cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology.

10p research12 23-04-2013 27 2 Download

Báo cáo khoa học: Charging of tRNA with non-natural amino acids at high pressure

We show a simple and reliable method of tRNA aminoacylation with natural, as well as non-natural, amino acids at high pressure. Such specific and noncognate tRNAs can be used as valuable substrates for protein engineering. Aminoacylation yield at high pressure depends on the chem-ical nature of the amino acid used and it is up to 10%.

10p inspiron33 25-03-2013 45 3 Download
Thermal Stability of RNA Phage Virus-Like Particles Displaying Foreign Peptides

Background: To be useful for genetic display of foreign peptides a viral coat protein must tolerate peptide insertions without major disruption of subunit folding and capsid assembly. The folding of the coat protein of RNA phage MS2 does not normally tolerate insertions in its AB-loop, but an engineered single-chain dimer readily accepts them as long as they are restricted to one of its two halves.

0p toshiba25 08-12-2011 46 3 Download
Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites

*Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, EH9 3JT, UK. †Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK. ‡Current address: Program in Genetics and Genomic Biology, Hospital for Sick Children, University Avenue, Toronto, Ontario M5G 1X8, Canada. §Current address: Facultad de Química, Cátedra de Inmunología, Universita de la Republica, Montevideo 11300, Uruguay. Correspondence: Rick M Maizels. E-mail: rick.maizels@ed.ac.

0p thulanh21 15-11-2011 57 3 Download