Genome editing

In ebook "Yeast protocols (Third edition)" the first eight chapters describe some basic protocols in yeast culture and genomic manipulation. Chapters 9–12 describe protocols that study certain organelles such as mitochondria and peroxisomes, and their functions in autophagy. Chapters 13– 19 are dedicated to some assays commonly used in yeast-based studies. These protocols can be potentially adapted to other organisms. The last two chapters describe two commonly used yeast-based protocols that are used to study not only yeast genes but also genes and gene products from other organisms.

325p dongmelo 27-05-2024 4 2   Download

Ebook "Mycobacteria protocols (Second edition)" updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

485p dongmelo 27-05-2024 8 3   Download

Ebook "Biology of the fungal cell: Volume VIII (2nd edition)" provides readers with contents including: the endomembrane system of the fungal cell; motile tubular vacuole systems; the fungal woronin body; a molecular and genomic view of the fungal cell wall; the cytoskeleton and polarized growth of filamentous fungi; polarised growth in fungi; signal transduction and morphogenesis in candida albicans;...

353p ladongphongthanh1008 22-04-2024 4 1   Download

CRISPR–Cas systems have provided revolutionary tools for genome editing. The discovery of Cas proteins with alternative activities has now enabled sensitive and robust tools for detecting nucleic acids. Recent reports harnessing these new CRISPR–Cas technologies display their potential for providing lowcost and practical diagnostic tools for pathogen and disease detection.

4p vibransone 28-03-2024 8 2   Download

Recent work has reported that the activation of the p53 pathway in primary cells hampers the efficiency of genome editing by CRISPR/Cas9 via induction of apoptosis or cell cycle arrest. These findings highlight the importance of wholly comprehending cellular responses to programmable nucleases in therapeutically relevant cell types to fulfill the promise of genome editing for therapeutic applications for human diseases.

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We present an assay to experimentally test the regulatory effects of genetic variants within transcripts using CRISPR/ Cas9 followed by targeted sequencing. We applied the assay to 32 premature stop-gained variants across the genome and in two Mendelian disease genes, 33 putative causal variants of eQTLs, and 62 control variants in HEK293T cells, replicating a subset of variants in HeLa cells.

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The rapid expansion of the available genomic data continues to greatly impact biomedical science and medicine. Fulfilling the clinical potential of genetic discoveries requires the development of therapeutics that can specifically modulate the expression of disease-relevant genes.

16p vioraclene 31-03-2024 2 2   Download

Genome-wide association studies (GWAS) have uncovered thousands of genetic variants that influence risk for human diseases and traits. Yet understanding the mechanisms by which these genetic variants, mainly noncoding, have an impact on associated diseases and traits remains a significant hurdle.

20p vibransone 28-03-2024 2 2   Download

Mutations in the DMD gene encoding dystrophin—a critical structural element in muscle cells— cause Duchenne muscular dystrophy (DMD), which is the most common fatal genetic disease. Clustered regularly interspaced short palindromic repeat (CRISPR)-mediated gene editing is a promising strategy for permanently curing DMD.

19p vibransone 28-03-2024 5 2   Download

Using BRCA1 and BRCA2 variants, which have consequences on PARP inhibitor sensitivity, and POLE variants, potential biomarkers of immunotherapy response, we developed a rapid functional assay based on CRISPR-Cas9 genome editing to determine the functional consequences of these variants having potentially direct implications on patients’ access to targeted therapies.

14p vibransone 28-03-2024 2 2   Download

Ebook Basic science in obstetrics AND gynaecology (4th edition): Part 1 includes contents: Structure and function of the genome, clinical genetics, embryology, fetal and placental physiology, applied anatomy, pathology,... and other contents.

174p longtimenosee03 30-01-2024 13 4   Download

Ebook "The prokaryotes: A handbook on the biology of bacteria (Volume 7: Proteobacteria: Delta and epsilon subclasses. Deeply rooting bacteria)" will make up this new fully revised and updated version. Compared to the second edition, this edition will contain 85% new contents, printed in color throughout. It will be ideally suited for research centers in academia and in the corporate world that need reliable and up-to-date information on the biology of the prokaryotic organisms.

1105p tachieuhoa 28-01-2024 7 2   Download

Ebook "Genome editing in animals: Methods and protocols" details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal.

258p lucchinguyen 28-12-2023 4 2   Download

Ebook "Bacteriophages: Methods and protocols (Volume 3)" expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing;...

311p lucchinguyen 28-12-2023 7 2   Download

Ebook "Functional genomics: Methods and protocols (Third edition)" seeks to aid scientists in establishing or extending technologies and techniques in their laboratories. Includes fully updated, cutting-edge techniques for the study of functional genomics, provides step-by-step detail essential for reproducible results, contains key implementation advice from the experts.

385p lucchinguyen 28-12-2023 5 2   Download

The increasing prevalence of cancer detection necessitated practical strategies to deliver highly accurate, beneficial, and dependable processed information together with experimental results. We deleted the cancer biomarker NOX4 using three novel genetic knockout (KO) methods. Homology-directed repair (HDR), Dual allele HITI (Du-HITI) and CRISPR-excision were utilized in this study.

15p vileonardodavinci 23-12-2023 5 3   Download

Part 2 book "Frontiers in aquaculture biotechnology" includes content: Recent advances in fish disease diagnosis, therapeutics, and vaccine development; principles of genome editing and its applications in fisheries, ultraviolet-B radiation, a potential threat to the aquatic organisms, and its mitigation; applications of biotechnology in aquaculture nutrition and fisheries; fish DNA barcoding - advances and challenges; recent advances in marine biotechnology, seaweed biotechnology implications to aquaculture,... and other contents.

173p oursky09 12-11-2023 4 3   Download

Part 2 book "Frontiers in aquaculture biotechnology" includes content: Principles of genome editing and its applications in fisheries; ultraviolet-B radiation, a potential threat to the aquatic organisms, and its mitigation; applications of biotechnology in aquaculture nutrition and fisheries; fish DNA barcoding - advances and challenges; recent advances in marine biotechnology; seaweed biotechnology implications to aquaculture.... and other contents.

141p oursky07 23-10-2023 6 1   Download

Part 1 book "The genetics of cattle" includes content: Systematics and phylogeny of cattle, genetic aspects of domestication, breeds of cattle, molecular genetics of coat colour variation, genetics of morphological traits and inherited disorders, cytogenetics and chromosome maps, bovine genomics, bovine immunogenetics, genetics of disease resistance,... and other contents.

328p oursky05 20-09-2023 5 1   Download

Part 2 book "The genetics of cattle" includes content: Developmental genetics, genetic improvement of dairy cattle, molecular genetics of milk protein production, genetics of fatty acid composition in bovine milk and beef, genetic improvement of beef cattle, genomic prediction and genome wide association studies in beef and dairy cattle,...and other contents.

314p oursky05 20-09-2023 6 1   Download