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GFP activities
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To characterize the cellular stress by n-alkanols with different alkyl chain lengths in Escherichia coli, we investigated how n-alkanols damage cell envelope permeability and whether they enhance the promoter activity of the envelope stress response regulator, σE , by using variants of green fluorescent protein (GFP)
14p
vicaptainmarvel
21-04-2023
5
2
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The aminated-silica nanoparticles with a positive zeta potential interact with GFP having a negative zeta potential via ionic force. GFP immobilized by the aminated silica nanoparticles greatly enhances (ca. 2 times) the bioluminescence intensity compared to free GFP. The lipase/aminated-silica nanoparticles catalyzed the transesterification reaction of 1-hexanol and ethyl acetate.
7p
spiritedaway36
28-11-2021
6
0
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Camphor is widely used in pharmacy, the food industry, and cosmetics. In this study, we evaluate inhibitory and cytotoxic effects of camphor in the fission yeast (Schizosaccharomyces pombe), which presents a unicellular model in mechanistic toxicology and cell biology. Low-dose camphor exposure (0.4 mg/mL) activated autophagy, which was shown by GFP-Atg8 dots and transcriptional upregulation of Atg6 (Beclin-1 ortholog). Autophagy was also confirmed by using autophagy-deficient cells, which showed reduction in GFP-Atg8 dot formation. However, high-dose camphor exposure (0.
9p
thiencuuchu
27-11-2021
8
1
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In the present study, attempts were made to produce biologically active recombinant mouse hephaestin as a secretory form tagged with green fluorescent protein (GFP), Hpsec-GFP. Plasmid expressing Hpsec-GFP was constructed and transfected into COS and CHO cells. The GFP aided the monitoring expression in real time to select the best conditions to maximise expression and provided a tag for purifying and analysing Hpsec-GFP. The protein had detectable oxidase activity as shown by in-gel and solution-based assays.
13p
thiencuuchu
27-11-2021
8
1
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Deletions of 6q15–16.1 are recurrently found in pediatric T-cell acute lymphoblastic leukemia (T-ALL). This chromosomal region includes the mitogen-activated protein kinase kinase kinase 7 (MAP3K7) gene which has a crucial role in innate immune signaling and was observed to be functionally and prognostically relevant in different cancer entities.
12p
vibaku2711
22-07-2020
8
1
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The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains.
12p
vihamax2711
21-04-2020
13
0
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In this study, systematic separation and subsequent pharmacological activity studies were carried out to identify cytotoxic natural products from the dried stems of Millettia pachyloba Drake. Five previously undescribed isoflavones, pachyvones A–E; one previously undescribed xanthone, pachythone A; and twenty-two known compounds were obtained. The structures of these compounds were assigned on the basis of 1D/2D NMR data and high-resolution electrospray ionization mass spectroscopy analysis.
11p
trinhthamhodang1
14-11-2019
19
1
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These vectors are totally able to express GFP protein without any inducer in B. subtilis strain 1012. Moreover, GFP expression level reached 11% of the total cellular proteins. Additionally, GFP activities are in the same range with the inducer-free vector.
9p
danhvi10
22-11-2018
18
1
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Fluorescence recovery after photobleaching (FRAP) and related techniques using green fluorescent protein (GFP)-tagged proteins are widely used to study the subcellular trafficking of proteins. It was concluded from these experiments that the cytokine-induced nuclear import of tyrosine-phos-phorylated (activated) signal transducer and activator of transcription 1 (STAT1) was rapid, while the constitutive shuttling of unphosphorylated STAT1 was determined to be inefficient.
12p
galaxyss3
21-03-2013
34
2
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In vivo reorganization of the actin cytoskeleton in leaves of Nicotiana tabacum L. transformed with plastin-GFP. Correlation with light-activated chloroplast responses
14p
panasonic06
25-12-2011
40
3
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