Glucose sensing

The work presented in this thesis illustrates strategies to improve the detection of glucose by NanoZymes. This was done by first determining the NanoZyme activity of Cu and bimetallic nanoparticles of Cu, followed by the application of this activity towards biosensing of glucose.

127p runthenight04 02-02-2023 4 2   Download

Dietary sugars regulate expression of the intestinal Na + / glucose cotransporter, SGLT1, inmany species.Using sheep intestine as a model, we showed that lumenal monosaccha-rides, both metabolisable and nonmetabolisable, regulate SGLT1 expression. This regulation occurs not only at the level of transcription, but also at the post-transcriptional level. Introduction of D-glucose andsome D-glucose ana-logues into ruminant sheep intestine resultedin 50-fold enhancement of SGLT1 expression.

12p tumor12 20-04-2013 34 2   Download

UhpC is a membrane-bound sensor protein inEscherichia colirequired for recognizing external glucose-6-phosphate (Glc6P) and induction of the transport protein UhpT. Recently, it was shown that UhpC is also able to transport Glc6P.In this studywe investigatedwhether these transport and sensing activities are obligatorily coupled in UhpC.We expressed a His-UhpC protein in a UhpC-deficientE. coli strain and verified that this construct does not alter the basic biochemical properties of the Glc6Psensor system....

8p tumor12 20-04-2013 58 2   Download

The addition of glucose toSaccharomyces cerevisiaecells causes reprogramming of gene expression. Glucose is sensed bymembrane receptorsaswell as (so far elusive) intracellular sensing mechanisms. The availability of four yeast strains that display different hexose uptake capacities allowed us to study glucose-induced effects at different glycolytic rates. Rapid glucose responses were observed in all strains able to take up glucose, consistent with intracellular sensing.

10p awards 05-04-2013 33 4   Download

The AMP-activated protein kinase is an evolutionarily conserved hetero-trimer that is important for metabolic sensing in all eukaryotes. The muscle-specific isoform of the regulatory c-subunit of the kinase, AMP-activated protein kinasec3, has a key role in glucose and fat metabolism in skeletal muscle, as suggested by metabolic characterization of humans, pigs and mice harboring substitutions in the AMP-binding Bateman domains ofc3.

10p galaxyss3 19-03-2013 37 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Research Glucose sensing in the pancreatic beta cell: a computational systems analysis...

44p toshiba17 28-10-2011 38 6   Download