Glucosyltransferase

Xem 1-5 trên 5 kết quả Glucosyltransferase

Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difﬁcile

Uridine-5¢-diphospho-1-a-D-glucose (UDP-Glc) is a common substrate used by glucosyltransferases, including certain bacterial toxins such as Toxins A and B from Clostridium diﬃcile. Fluorescent analogs of UDP-Glc have been prepared for use in our studies of the clostridial toxins. These compounds are related to the methylanthraniloylATP compounds commonly used to probe the chemistry of ATP-dependent enzymes. The reaction of excess methylisatoic anhydride with UDP-Glc in aqueous solution yields primarily the 2¢ and 3¢ isomers of methylanthraniloyl-UDPGlc (MUG).

8p research12 01-06-2013 43 4 Download

Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase

Sugar conjugation is a major pathway for the inactivation and excretion of both endogenous and exogenous compounds. We report here the molecular cloning and functional characterization of a phenol UDP-glucosyltransferase (UGT) from the silkworm, Bombyx mori, which was named BmUGT1. The complete cDNA clone is 1.6 kb, and the gene is expressed in several tissues of ﬁfth-instar larvae, including fat body, midgut, integument, testis, silk gland and haemocytes. The predicted protein comprises 520 amino acids and has 30% overall amino-acid identity with other members of the UGT family.

7p research12 01-06-2013 39 4 Download

Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis

Glycogen phosphorylases (GPs) constitute a family of widely spread catabolica1,4-glucosyltransferases that are active as dimers of two identical, pyridoxal 5¢-phosphate-containing subunits. In GP fromCorynebacterium callunae, physiological concentrations of phosphate are required to inhibit dissociation of protomers and cause a 100-fold increase in kinetic stability of the functional quarternary structure.

11p fptmusic 16-04-2013 35 2 Download
Báo cáo khoa học: A kinetic study of sugarcane sucrose synthase

The kinetic data on sugarcane (Saccharumspp. hybrids) sucrose synthase (SuSy, UDP-glucose: D-fructose 2-a-D-glucosyltransferase, EC 2.4.1.13) are limited. We character-ized kinetically a SuSy activity partially purified from sugarcane variety N19 leaf roll tissue. Primary plot analysis and product inhibition studies showed that a compulsory order ternary complex mechanism is followed, with UDP binding first and UDP-glucose dissociating last from the enzyme.

0p awards 05-04-2013 56 2 Download
báo cáo khoa học: " Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation

16p panasonic06 25-12-2011 50 3 Download