Glycolytic enzymes

- Polysaccharides are macromolecules, polymers with a few hundred to a few thousand monosaccharides joined by glycosidic linkages. - Some polysaccharides serve as storage material, hydrolyzed as needed to provide sugar for cells. - Other polysaccharides serve as building material for structures that protect the cell or the whole organism. - The architecture and function of a polysaccharide are determined by its sugar monomers and by the positions of its glycosidic linkages.

118p zingzing09 21-10-2012 54 8   Download

We previously reported that GTS1 is involved in regulating ultradian oscillations of the glycolytic pathway induced by cyanide in cell suspensions as well as oscillations of energy metabolism in aerobic continuous cultures. Here, we screened a yeast cDNA library for proteins that bind to Gts1p using the yeast two-hybrid system and cloned multiple TDH cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

10p research12 01-06-2013 41 3   Download

In this article, we report the results of an analysis of the glycolytic enzyme enolase (2-phospho-D-glycerate hydro-lase) of Trypanosoma brucei. Enolase activity was detected in both bloodstream-form and procyclic insect-stage try-panosomes, although a 4.5-fold lower specific activity was found in the cultured procyclic homogenate. Subcellular localization analysis showed that the enzyme is only pre-sent in the cytosol.

9p tumor12 20-04-2013 29 3   Download

It has been shown previously in various organisms that the peroxin PEX14 is a component of a docking complex at the peroxisomalmembrane,where it is involved in the import of matrix proteins into the organelle after their synthesis in the cytosol and recognition by a receptor. Here we present a characterization of theTrypanosoma bruceihomologue of PEX14. It is shown that the protein is associated with gly-cosomes,the peroxisome-like organelles of trypanosomatids in which most glycolytic enzymes are compartmentalized....

9p fptmusic 16-04-2013 47 2   Download

A pathway intermediate is said to be ‘channeled’ when an intermediate just made in a pathway has a higher probability of being a substrate for the next pathway enzyme compared with a molecule of the same species from the aqueous cytoplasm. Channeling is an important phenomenon because it might play a significant role in the regulation of metabolism.

10p awards 06-04-2013 47 4   Download

The synthesis of ATP in the human parasiteEntamoeba histolyticais car-ried out solely by the glycolytic pathway. Little kinetic and structural infor-mation is available for most of the pathway enzymes.

17p awards 06-04-2013 34 3   Download

Depolarization and repolarization phases (D and R phases, respectively) of mitochondrial potential fluctuations induced by photoactivation of the fluorescent probe tetramethylrhodamine methyl ester (TMRM) were ana-lyzed separately and investigated using specific inhibitors and substrates. The frequency of R phases was significantly inhibited by oligomycin and aurovertin (mitochondrial ATP synthase inhibitors), rotenone (mitochond-rial complex I inhibitor) and iodoacetic acid (inhibitor of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase)....

11p awards 06-04-2013 48 2   Download

Control analysis of the glycolytic flux was carried out in two fast-growth tumor cell types of human and rodent origin (HeLa and AS-30D, respect-ively). Determination of the maximal velocity (Vmax) of the 10 glycolytic enzymes from hexokinase to lactate dehydrogenase revealed that hexokin-ase (153–306 times) and phosphfructokinase-1 (PFK-1) (22–56 times) had higher over-expression in rat AS-30D hepatoma cells than in normal freshly isolated rat hepatocytes.

14p inspiron33 26-03-2013 48 5   Download

Protein glycation by methylglyoxal is a nonenzymatic post-translational modification whereby arginine and lysine side chains form a chemically heterogeneous group of advanced glycation end-products. Methylglyoxal-derived advanced glycation end-products are involved in pathologies such as diabetes and neurodegenerative diseases of the amyloid type.

15p inspiron33 23-03-2013 51 4   Download

In several archaea of the Euryarchaeota, the glycolytic flux proceeds through a modified version of the Embden–Meyerhof pathway, where the phosphofructokinase and glucokinase enzymes use ADP as the phosphoryl donor. These enzymes are homologous to each other. In the hyperthermo-philic methanogenic archaeon

12p galaxyss3 07-03-2013 42 2   Download

The in vitro Entamoeba histolytica pyruvate:ferredoxin oxidoreductase (EhPFOR) kinetic properties and the effect of oxidative stress on glycolytic pathway enzymes and fluxes in live trophozoites were evaluated.EhPFOR showed a strong preference for pyruvate as substrate over other oxoacids.

14p viettel02 19-02-2013 38 3   Download

Loose interaction between the glycolytic enzymes glyceraldehyde-3-phos-phate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) was visualized in living CHO-K1 cells by fluorescence resonance energy transfer (FRET), using time-domain fluorescence lifetime imaging microscopy.

9p mobifone23 18-01-2013 44 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes...

14p thulanh14 17-10-2011 52 4   Download