Glycosylation and glycoproteins

TheX-raystructureofnativecellobiohydrolase IB(CBHIB) from the filamentous fungus Talaromyces emersonii,PDB 1Q9H,was solved to2.4 A ˚ bymolecular replacement. 1Q9H is a glycoprotein that consists of a large, single domain with dimensions of
60 A ˚ ·40 A ˚ ·50 A ˚ andanoverall b-sandwich structure, the characteristic fold of Family 7 glycosyl hydrolases (GH7). It is the first structure of a native glycoprotein and cellulase fromthis thermophilic eukaryote.

12p awards 05-04-2013 36 2   Download

Mass spectrometric studies on the N-linked glycans of aminopeptidase 1 from Manduca sextahave revealed unusual structures not previously observed on any insect glycoprotein. Structure elucidation of these oligosaccha-rides was carried out by high-energy collision-induced dissociation (CID) using a matrix-assisted laser desorp-tion/ionization time-of-flight/time-of-flight (MALDI-TOF/ TOF) tandem mass spectrometer.

18p awards 05-04-2013 36 4   Download

Gas1p is a glycosylphosphatidylinositol-anchored plasma membrane glycoprotein ofSaccharomyces cerevisiaeand is a representative of Family GH72 of glycosidases/transgly-cosidases, which also includes proteins from human fungal pathogens. Gas1p, Phr1-2p from Candida albicansand Gel1p fromAspergillus fumigatushave been shown to be b-(1,3)-glucanosyltransferases required for proper cell wall assembly andmorphogenesis. Gas1p is organized into three modules: a catalytic domain; a cys-richdomain; andahighly O-glycosylated serine-rich region. ...

11p awards 05-04-2013 47 4   Download

There are different glycosylated proteins in snake venoms, but no glycosylated representatives of a large family of three-fingered toxins have previously been detected. A new glycoprotein was isolated from the venom of the Thai cobra Naja kaouthia. MALDI MS of the glycoprotein contained an array of peaks in the range from
8900 to
9400 Da indicating its microheterogeneity. Carbohy-drate analysis showed the presence of mannose, galactose, N-acetylglucosamine, fucose and neuraminic acid.

10p dell39 03-04-2013 38 2   Download

GM-CSF is one of several naturally occurring glycoproteins that regulate leukocyte production, migration and function. It has been produced in different cell types, with different properties that depend on the production process used. The purpose of this work was to characterize the recombinant human GM-CSF from an engineered Chinese hamster ovary cell line grown in suspension and as adherent culture for the identification of the glycosylation sites and the defi-nition of the glycosidic moiety, including the degree of site occupancy....

13p dell39 03-04-2013 46 4   Download

Lactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. To elucidate the role ofN-linked glycosylation in protection of LF against proteolysis, we compared the tryptic susceptibility of human LF (hLF) variants from human milk, expressed in human 293(S) cells or in the milk of transgenic mice and cows. The analysis revealed that recombinant hLF (rhLF) with mutations Ile130fiThr and Gly404fiCys was about twofold more susceptible than glycosylated and unglycosylated variants with the naturally occurring Ile130 and Gly404....

7p dell39 03-04-2013 41 5   Download

Urinary glycoproteins are important inhibitors of calcium oxalate crystal-lization and adhesion of crystals to renal cells, both of which are key mech-anisms in kidney stone formation. This has been attributed to glycosylation of the proteins. In South Africa, the black population rarely form stones (incidence

14p inspiron33 25-03-2013 30 3   Download

Mucin, a major component of mucus, is a highly O-glycosylated, high-molecular-mass glycoprotein extensively involved in the physiology of gastrointestinal mucosa. To detect and characterize mucins derived from site-specific mucous cells, we developed a monoclonal antibody, designated PGM34, by immunizing a mouse with purified pig gastric mucin.

16p galaxyss3 21-03-2013 36 4   Download