Green fluorescent protein
-
Ebook "Fluorescent proteins II: Application of fluorescent protein technology" highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
287p manmanthanhla0201 26-02-2024 2 1 Download
-
Ebook "Analytical ultracentrifugation of polymers and nanoparticles" is divided into chapters concerning instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory.
249p manmanthanhla0201 27-02-2024 4 2 Download
-
Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, Green Fluorescent Protein (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of a synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli).
10p vimulcahy 18-09-2023 4 3 Download
-
To characterize the cellular stress by n-alkanols with different alkyl chain lengths in Escherichia coli, we investigated how n-alkanols damage cell envelope permeability and whether they enhance the promoter activity of the envelope stress response regulator, σE , by using variants of green fluorescent protein (GFP)
14p vicaptainmarvel 21-04-2023 5 2 Download
-
Fish are remarkably diverse in repertoires of visual opsins by gene duplications. Differentiation of their spatiotemporal expression patterns and absorption spectra enables fine-tuning of feature detection in spectrally distinct regions of the visual field during ontogeny.
12p vinarcissa 21-03-2023 5 1 Download
-
Different degrees of protein purity have been observed in immobilized metal affinity chromatography ranging from extremely high purity to moderate and low purity. It has been hypothesized that the host cell protein composition and the metal ligands are factors governing the purity of a protein obtained after immobilized metal affinity chromatography (IMAC).
10p viginny 23-12-2022 9 3 Download
-
The aminated-silica nanoparticles with a positive zeta potential interact with GFP having a negative zeta potential via ionic force. GFP immobilized by the aminated silica nanoparticles greatly enhances (ca. 2 times) the bioluminescence intensity compared to free GFP. The lipase/aminated-silica nanoparticles catalyzed the transesterification reaction of 1-hexanol and ethyl acetate.
7p spiritedaway36 28-11-2021 6 0 Download
-
The characteristics of synthesized nanoparticles depend on the source of the plant extracts because of different combinations and concentrations of organic reducing agents present in the extracts (Mukunthan and Balaji, 2012).
178p spiritedaway36 26-11-2021 7 2 Download
-
In the present study, attempts were made to produce biologically active recombinant mouse hephaestin as a secretory form tagged with green fluorescent protein (GFP), Hpsec-GFP. Plasmid expressing Hpsec-GFP was constructed and transfected into COS and CHO cells. The GFP aided the monitoring expression in real time to select the best conditions to maximise expression and provided a tag for purifying and analysing Hpsec-GFP. The protein had detectable oxidase activity as shown by in-gel and solution-based assays.
13p thiencuuchu 27-11-2021 8 1 Download
-
Glucocorticoid-associated osteonecrosis is an intractable condition, making the establishment of preventative strategies of particular importance. Recently various studies using mesenchymal stem cells (MSC) have been conducted.
7p vitennessee2711 02-02-2021 8 1 Download
-
The tauGFP reporter fusion protein is produced nearly ubiquitously by the TgTP6.3 transgene in TP6.3 mice and its localisation to microtubules offers some advantages over soluble GFP as a lineage marker. However, TgTP6.3Tg/Tg homozygotes are not viable and TgTP6.3Tg/− hemizygotes are smaller than wild-type.
16p viwyoming2711 16-12-2020 10 1 Download
-
Analysis of cellular processes with microscopic bright field defocused imaging has the advantage of low phototoxicity and minimal sample preparation. However bright field images lack the contrast and nuclei reporting available with florescent approaches and therefore present a challenge to methods that segment and track the live cells.
10p vikentucky2711 26-11-2020 10 0 Download
-
Bài viết trình bày ứng dụng kỹ thuật miễn dịch huỳnh quang kết hợp phân tích hình ảnh nội hàm cao để phát hiện và định lượng sự chuyển vị của tiểu đơn vị NF-κB p65 liên kết protein huỳnh quang xanh (green fluorescent protein, GFP) kích hoạt bởi cytokine trên dòng tế bào ung thư cổ tử cung HeLa.
9p kethamoi7 15-08-2020 86 4 Download
-
Replication-competent adenoviruses (Ad) produced cytotoxic effects on infected tumors and have been examined for the clinical applicability. A biomarkers to predict the cytotoxicity is valuable in a clinical setting.
12p vimanama2711 30-07-2020 6 2 Download
-
Anaplastic thyroid cancer (ATC) is one of the most aggressive of all solid tumors for which no effective therapies are currently available. Oncolytic Newcastle disease virus (NDV) has shown the potential to induce oncolytic cell death in a variety of cancer cells of diverse origins.
10p vimanila2711 29-06-2020 8 1 Download
-
To determine the titer of baculovirus stock accurately based on green fluorescent protein (GFP) reporter, a recombinant baculovirus encoding FLAG-tagged protein and GFP reporter as bicistronic messenger was constructed. The bicistronic messenger RNA was transcribed using polyhedrin promoter and translated in infected Sf21 insect cells. The GFP reporter gene was expressed incap independent manner utilizing internal ribosome entry site (IRES) which helped in titration of baculovirus using end point titration method.
9p quenchua6 15-06-2020 10 1 Download
-
The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms. Here, the CRISPR/ Cas9 system is shown to be effective in soybean by knocking-out a green fluorescent protein (GFP) transgene and modifying nine endogenous loci.
10p viuchiha2711 21-04-2020 12 1 Download
-
A superior Green Fluorescent Protein (GFP) mutant, known as superfolder GFP (sfGFP), is more soluble, faster folding, and is the brightest of the known GFP mutants. This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purification in Tetrahymena thermophila.
9p viuchiha2711 21-04-2020 10 0 Download
-
The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains.
12p vihamax2711 21-04-2020 13 0 Download
-
The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and induce apoptosis and lead to the termination of protein expression.
8p vihamax2711 21-04-2020 19 0 Download