Green fluorescent protein

Luận văn nghiên cứu với mục tiêu nhằm mục đích làm tiền đề cho các nghiên cứu tiếp theo luận văn tiến hành nghiên cứu xây dựng qui trình thu nhận tế bào tiền thân nội mô từ máu cuống rốn người và thiết lập tế bào tiền thân nội mô từ máu cuống rốn người biểu hiện gen gfp để theo dõi đánh giá chức năng của chúng về khả năng sửa chữa nội mạch trong mô hình chuột thiếu máu chi cục bộ và thiếu máu cơ tim cục bộ. Để biết rõ hơn về nội dung chi tiết, mời các bạn cùng tham khảo.

78p dangthingocthuy96 09-01-2017 92 12   Download

Tham khảo luận văn - đề án 'chuyển gen gfp (green fluorescent protein) vào tế bào vi khuẩn pseudomonas fluorescens bằng phương pháp tiếp hợp ba thành phần', luận văn - báo cáo, khoa học tự nhiên phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

81p canhchuon_1 20-06-2013 138 28   Download

Adenosine 5’-triphosphate (ATP) là hợp chất cao năng quan trọng nhất, giữ vai trò cung cấp năng lƣợng trong mọi tế bào sống. Bởi vì trong phân tử ATP có hai liên kết phosphate cao năng, năng lƣợng tự do sẽ đƣợc giải phóng khi ATP bị thủy phân thành Adenosine 5’-diphosphate (ADP) và một phosphate vô cơ (Pi), hoặc Adenosine monophosphate (AMP) và pyrophosphate (PPi). Do đó, phần lớn các hoạt động sống nhƣ vận chuyển, hấp thu dinh dƣỡng, sinh tổng hợp các chất, phân chia tế bào … đều sử dụng ATP nhƣ nguồn năng lƣợng trực...

67p canhchuon_1 19-06-2013 88 11   Download

Wehave identifiedanovelmammaliangene, termednicolin1 gene (NICN1), that is present in human, dog and mouse, whereas it is absent from the available genome sequences of nonmammalian organisms. TheNICN1gene consists of six exons and spans about 6 kb of genomic DNA. It encodes a 213 amino acid protein that does not belong to any known protein family. Experiments using green fluorescent protein (GFP)-taggednicolin1 fusionproteins indicate that nicolin1 is a nuclear protein.

6p tumor12 22-04-2013 51 2   Download

The ultimate step in Alzheimer’s disease Abgeneration involves c-secretase, which releases Abfrom its membrane-bound precursor. Asimilar presenilin-dependent proteolytic activity is implicated in the release of theNotch intracellular domain. We have developed a novel assay forc-secretase activity based on green fluorescent protein detection. This involves cotransfection of a substrate-activator based on the amyloid precursor protein or the Notch sequence and a fluorescent reporter gene.

12p tumor12 20-04-2013 31 4   Download

Oxysterol binding protein (OSBP) and its homologs have been shown to regulate lipid metabolism and vesicular transport. However, the exact molecular function of individual OSBP homologs remains uncharacterized. Here we demonstrate that the yeast OSBP homolog, Osh6p, bound phos-phatidic acid and phosphoinositides via its N-terminal half containing the conserved OSBP-related domain (ORD). Using a green fluorescent protein fusion chimera, Osh6p was found to localize to the cytosol and patch-like or punctate structures in the vicinity of the plasma membrane....

13p fptmusic 12-04-2013 52 3   Download

Salt-inducible kinase-1 (SIK1) is phosphorylated at Ser577 by protein kinase A in adrenocorticotropic hormone-sti-mulated Y1 cells, and the phospho-SIK1 translocates from the nucleus to the cytoplasm. The phospho-SIK1 is dephosphorylated in the cytoplasmand re-enters thenucleus several hours later. By using green-fluorescent protein-tag-ged SIK1 fragments, we found that a peptide region (586– 612) was responsible for the nuclear localization of SIK1. Theregionwas namedthe
RK-rich region because of its Arg- and Lys-rich nature. ...

13p awards 05-04-2013 41 4   Download

Phosphorylationof the transit peptideof several chloroplast-targeted proteins enables the binding of 14-3-3 proteins. The complex that forms, together with Hsp70, has been demonstrated to be an intermediate in the chloroplast pro-tein import pathwayin vitro[May, T. &Soll, J. (2000)Plant Cell12, 53–63]. In this paper we report that mutagenesis (in order to remove the phosphorylation site) of the transit peptide of the small subunit of ribulose bisphosphate carb-oxylase/oxygenase did not affect its ability to target green fluorescent protein to chloroplastsin vivo....

8p dell39 03-04-2013 51 4   Download

Pigments homologous to the green fluorescent protein (GFP) contribute up to 14% of the soluble protein content of many anthozoans. Maintenance of such high tissue levels poses a severe energetic penalty to the animals if protein turnover is fast. To address this as yet unexplored issue, we estab-lished that the irreversible green-to-red conversion of the GFP-like pig-

10p galaxyss3 21-03-2013 42 3   Download

For a variety of coral species, we have studied the molecular origin of their coloration to assess the contributions of host and symbiont pigments. For the corals Catalaphyllia jardineiand an orange-emitting color morph of Lobophyllia hemprichii, the pigments belong to a particular class of green fluorescent protein-like proteins that change their color from green to red upon irradiation with400 nm light.

8p galaxyss3 21-03-2013 37 2   Download

Fluorescence recovery after photobleaching (FRAP) and related techniques using green fluorescent protein (GFP)-tagged proteins are widely used to study the subcellular trafficking of proteins. It was concluded from these experiments that the cytokine-induced nuclear import of tyrosine-phos-phorylated (activated) signal transducer and activator of transcription 1 (STAT1) was rapid, while the constitutive shuttling of unphosphorylated STAT1 was determined to be inefficient.

12p galaxyss3 21-03-2013 39 4   Download

TRPM4b is a Ca 2+ -activated, voltage-dependent monovalent cation chan-nel that has been shown to act as a negative regulator of Ca 2+ entry and to be involved in the generation of oscillations of Ca 2+ influx in Jurkat T-lymphocytes. Transient overexpression of TRPM4b as an enhanced green fluorescence fusion protein in human embryonic kidney (HEK) cells resulted in its localization in the plasma membrane, as demonstrated by confocal fluorescence microscopy.

10p galaxyss3 21-03-2013 34 3   Download

To measure mechanical stress in real time, we designed a fluorescence reso-nance energy transfer (FRET) cassette, denoted stFRET, which could be inserted into structural protein hosts. The probe was composed of a green fluorescence protein pair, Cerulean and Venus, linked with a stablea-helix.

16p galaxyss3 07-03-2013 50 6   Download

Human heart LIM protein (hhLIM) is a newly cloned protein.In vitro analyses showed that green fluorescent protein (GFP)-tagged hhLIM pro-tein accumulated in the cytoplasm of C2C12 cells and colocalized with F-actin, indicating that hhLIM is an actin-binding protein in C2C12 cells.

11p media19 06-03-2013 49 1   Download

Plastid envelope DNA-binding protein (PEND) is a DNA-binding protein with a chloroplast basic region-zipper domain at its N-terminus and a transmembrane domain at its C-terminus. The localization of PEND to the inner envelope membrane was demonstrated in a targeting experiment using isolated membranes and green fluorescent protein-tagged fusion pro-teins.

11p vinaphone15 27-02-2013 33 3   Download

Fluorescent proteins have become essential tools in molecular and biologi-cal applications. Here, we present a novel fluorescent protein isolated from warm water coral, Cyphastrea microphthalma. The protein, which we named vivid Verde fluorescent protein (VFP), matures readily at 37C and emits bright green light.

12p mobifone23 21-01-2013 53 4   Download

Minor structural proteins of mouse polyomavirus (MPyV) are essential for virus infection. To study their properties and possible contributions to cell death induction, fusion variants of these proteins, created by linking enhanced green fluorescent protein (EGFP) to their C- or N-termini, were prepared and tested in the absence of other MPyV gene products, namely the tumor antigens and the major capsid protein, VP1.

14p mobifone23 18-01-2013 44 4   Download

TheOryza sativalectin, abbreviated Orysata, is a mannose-specific, jacalin-related lectin expressed in rice plants after exposure to certain stress condi-tions. Expression of a fusion construct containing the rice lectin sequence linked to enhanced green fluorescent protein in Bright Yellow 2 tobacco cells revealed that Orysata is located in the nucleus and the cytoplasm of the plant cell, indicating that it belongs to the class of nucleocytoplasmic jacalin-related lectins.

16p cosis54 05-01-2013 51 5   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule

3p panasonic01 13-12-2011 52 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Development of a method for screening short-lived proteins using green fluorescent protein...

8p thulanh19 09-11-2011 53 4   Download