Haploid induction
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Anther culture is an effective breeding method for improving desirable traits, since it allows fast achievement of genetically homozygous lines. The present studies were carried out to evaluate the response of flue-cured tobacco anthers for high frequency pollen embryo induction in different culture media, and the effects of colchicine treatment on tobacco chromosome doubling. The results showed that different media caused significant changes in pollen embryo induction, in which the most obvious change was found in B medium with induction rates of 23.28%, 26.64%, and 28.
9p tudichquannguyet 29-11-2021 5 1 Download
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Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Production of pure lines by DH induction provides a new way to achieve homozygosity earlier in B.napus.
13p vijichea2711 28-05-2021 19 1 Download
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Doubled haploid (DH) lines produced via in vivo haploid induction have become indispensable in maize research and practical breeding, so it is important to understand traits characteristics in DH and its corresponding haploids which derived from each DH lines.
15p vihashirama2711 21-05-2020 12 0 Download
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Microspore embryogenesis describes a stress-induced reprogramming of immature male plant gametophytes to develop into embryo-like structures, which can be regenerated into doubled haploid plants after whole genome reduplication. This mechanism is of high interest for both research as well as plant breeding.
16p vihashirama2711 21-05-2020 14 1 Download
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Sporophytic plants having gametophytic chromosome number are termed as haploid. These plants can be produced either spontaneously in nature or by in vitro or in vivo induction technique. By doubling chromosome number of haploid either spontaneously or artificially, double haploid (DH) could be produced. Double haploid are homozygous and this homozygosity is achieved in one generation by using DH production systems. This production system is used for fixation of heterosis. Genetic map construction is relatively easy with DH population.
15p nguaconbaynhay5 16-05-2020 7 1 Download
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In vivo haploid induction (HI) based on Stock6-derived inducer lines has been the most prevalent means of producing haploids. Nevertheless, the biological mechanism of HI is not fully understood, the twinembryo kernels had been found during haploid induction, which may provide potential evidence for the abnormal double fertilization during HI.
9p vishikamaru2711 29-04-2020 12 1 Download
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In the present study, anther of three varieties and their cross combinations of Brassica campestris, namely HPBS-1, KBS-3, HPKM-04-1, HPBS-1 x HPKM-04-1 and KBS-3 x HPKM-04-1 were cultured in vitro to observe their callus induction frequency. The effect of two basal media i.e. MS and N6, two different sucrose concentrations i.e. 3% and 4%, three combinations of growth hormones viz. HM1, HM2, HM3 and their callus induction frequency were analyzed by CPCS software. Out of all factors and their interactions, the genotype HPBS-1 performed better in N6 medium with HM2 [0.5 mg/l 2, 4-D + 1.
10p nguathienthan2 19-12-2019 16 1 Download
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Protocols for haploid induction in cucurbits are based on pollination with irradiated pollen, but the induction frequency is low and the majority of obtained embryos are zygotic. The longevity of Cucurbita pepo L. pollen is short even under natural conditions; following irradiation, germinability is decreased even further.
7p caplock2711 22-02-2019 19 0 Download
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Doubled haploid induction is one of the available methods normally used for sugar beet breeding. Gynogenic haploid explants of sugar beet induced with 1 or 2 mg L–1 6-benzylaminopurine were treated with 5 g L–1 colchicine, then subcultured on a solidified MS medium plus 0.2 mg L–1 kinetin.
9p caplock2711 21-02-2019 10 1 Download
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Effects of culture media and genotypes of water chestnut (Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with 0.5 mg/l BA, 0.5 mg/l NAA and 0.1 mg/l GA3 were suitable for callus induction.
5p vitamix 15-02-2019 12 1 Download
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The aim of this study was to determine the androgenic capacity of some eggplant (Solanum melongena L.) varieties and breeding lines, and to establish the effectiveness of androgenesis induction in the less responsive genotypes by crossing with more responsive genotypes that have a high ability for haploid embryo formation.
7p sony2711 01-02-2019 44 2 Download
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The combined effect of anther incubation time on CP induction medium (12, 14, and 16 days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the effectiveness of androgenesis was investigated in 17 genotypes of Capsicum grown in Poland.
7p sony2711 01-02-2019 28 1 Download
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The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture.
15p nutifooddau 27-01-2019 15 2 Download
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This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus induction rate both on solid and in liquid medium.
8p aquaman27 15-01-2019 28 1 Download
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In vivo induction of parthenogenesis using an irradiated pollen technique provides successful results in melon and accelerates breeding by shortening the time for obtaining 100% homozygous lines. Gamma rays (cobalt-60) are usually used for the pollen irradiation, although it is difficult to find a suitable source for irradiation.
8p danhdanh11 09-01-2019 20 2 Download
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Breeding based on doubled-haploid approaches has recently become a common tool for accelerating crop improvement in many plant species. However, many plant species do not have a reliable method for haploid induction. A promising new approach involving centromere engineering has recently been proposed to overcome this limitation.
6p danhdanh11 11-01-2019 17 1 Download
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This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males.
5p danhdanh11 11-01-2019 23 2 Download