Hatching enzyme

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Báo cáo khoa học: Purification and gene cloning ofFundulus heteroclitus hatching enzyme A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts,Fundulus heteroclitusand medaka Oryzias latipes

Two cDNA homologues of medaka hatching enzyme)high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE) – were cloned from Fundulus heteroclitusembryos. Amino acid sequences of the mature forms ofFundulusHCE (FHCE) and LCE (FLCE) were 77.9% and 63.3% iden-tical to those of medaka HCE and LCE, respectively. In addition, phylo-genetic analysis clearly showed that FHCE and FLCE belonged to the clades of HCE and LCE, respectively.

12p fptmusic 11-04-2013 53 3 Download

Báo cáo khoa học: Hatching enzyme of the ovoviviparous black rockﬁsh Sebastes schlegelii – environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene

The hatching enzyme of oviparous euteleostean fishes consists of two metalloproteases: high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE). They cooperatively digest the egg envelope (chorion) at the time of embryo hatching. In the present study, we investigated the hatching of embryos of the ovoviviparous black rockfish Sebastes schlegelii.

15p galaxyss3 07-03-2013 39 3 Download

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion

There are two hatching enzyme homologues in the zebrafish genome: zebrafish hatching enzymeZHE1andZHE2. Northern blot and RT-PCR analysis revealed that ZHE1 was mainly expressed in pre-hatching embryos, whereasZHE2was rarely expressed. This was consistent with the results obtained in an experiment conducted at the protein level, which demonstrated that one kind of hatching enzyme, ZHE1, was able to be purified from the hatching liquid.

13p vinaphone15 28-02-2013 53 2 Download
Báo cáo khoa hoc : Hatching enzyme of Japanese eel Anguilla japonica and the possible evolution of the egg envelope digestion mechanism

We purified eel hatching enzyme (EHE) from the hatching liquid of Japa-nese eelAnguilla japonicabelonging to Elopomorpha to a single band on SDS⁄PAGE. TOF-MS analysis revealed that the purified EHE contained several isozymes with similar molecular masses.

13p cosis54 09-12-2012 47 4 Download