Báo cáo khoa học: Purification and gene cloning ofFundulus heteroclitus hatching enzyme A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts,Fundulus heteroclitusand medaka Oryzias latipes
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Two cDNA homologues of medaka hatching enzyme)high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE) – were cloned from Fundulus heteroclitusembryos. Amino acid sequences of the mature forms ofFundulusHCE (FHCE) and LCE (FLCE) were 77.9% and 63.3% iden-tical to those of medaka HCE and LCE, respectively. In addition, phylo-genetic analysis clearly showed that FHCE and FLCE belonged to the clades of HCE and LCE, respectively.
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