Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana
48
lượt xem 4
download
lượt xem 4
download
Download
Vui lòng tải xuống để xem tài liệu đầy đủ
The present study reports the first purification and kinetic characterization of two plant arogenate dehydrogenases (EC 1.3.1.43), an enzyme that catalyses the oxidative decarboxylation of arogenate into tyrosine in presence of NADP. The twoArabidopsis thalianaarogenate dehy-drogenases TyrAAT1 and TyrAAT2 were overproduced in Escherichia coliand purified to homogeneity. Bio-chemical comparison of the two forms revealed that at low substrate concentration TyrAAT1 is four times more efficient in catalyzing the arogenate dehydrogenase reac-tion than TyrAAT2. ...
Chủ đề:
Bình luận(0) Đăng nhập để gửi bình luận!
CÓ THỂ BẠN MUỐN DOWNLOAD