Báo cáo khoa học: Purification of phosphoproteins by immobilized metal affinity chromatography and its application to phosphoproteome analysis
39
lượt xem 3
download
lượt xem 3
download
Download
Vui lòng tải xuống để xem tài liệu đầy đủ
Prefractionation procedures facilitate the identification of lower-abundance proteins in proteome analysis. Here we have optimized the conditions for immobilized metal affinity chromatography (IMAC) to enrich for phos-phoproteins. The metal ions, Ga(III), Fe(III), Zn(II), and Al(III), were compared for their abilities to trap phosphoproteins; Ga(III) was the best.
Chủ đề:
Bình luận(0) Đăng nhập để gửi bình luận!
CÓ THỂ BẠN MUỐN DOWNLOAD