Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.)
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A new enzyme,S-adenosyl-L-methionine:flavonoid 4¢-O-methyltransferase (EC 2.1.1.-)(F 4¢-OMT), has been puri-fied 1 399-fold from the tissues of carnation (Dianthus caryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxysubstituent in4¢-positionof theflavones, flavanones and isoflavones in the presence ofS-adenosyl-L-methionine. A high affinity for the flavone kaempferol was observed (Km¼1.7lM;Vmax¼95.2lmolÆmin )1 Æmg )1 ), while other 4¢-hydroxylated flavonoids proved likewise to be suitable substrates....
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