Heavy chain antibodies

Nanobodies are single-domain antibodies that contain the unique structural and functional properties of naturally-occurring heavy chain in camelidae. As a novel class of antibody, they show many advantages compared with traditional antibodies such as smaller size, higher stability, improved specificity, more easily expressed in microorganisms. These unusual hallmarks make them as promising tools in basic research and clinical practice.

5p vilarryellison 29-10-2021 7 1   Download

In this paper we optimized our previous strategy by generating heavy chain antibodies (HCAbs) to overcome the deficiencies of single domain antibodies. Here we identified and characterized a heavy chain antibody (HCAb2) that targets cell surface HSP90 antigen on breast tumor cells but not normal cells.

19p viamsterdam 18-09-2020 7 0   Download

Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six major biological warfare agents by the Centers for Disease Control.

14p viuchiha2711 21-04-2020 12 0   Download

Catalytic antibodies capable of digesting crucial proteins of pathogenic bac-teria have long been sought for potential therapeutic use. Helicobacter pyloriurease plays a crucial role for the survival of this bacterium in the highly acidic conditions of human stomach. The HpU-9 monoclonal anti-body (mAb) raised againstH. pyloriurease recognized thea-subunit of the urease, but only slightly recognized theb-subunit. However, when isolated both the light and the heavy chains of this antibody were mostly bound to the b-subunit....

9p fptmusic 11-04-2013 44 2   Download

This study describes the construction of a library of single-chain antibody fragments (scFvs) from a single human donor by individual amplification of all heavy and light variable domains (1.1·10 8 recombinants). The lib-rary was panned using the phage display technique, which allowed selection of specific scFvs (3F and C1) capable of recognizing Cn2, the major toxic component of Centruroides noxiusscorpion venom.

11p awards 06-04-2013 47 2   Download

To address questions of protein stability, researchers have increasingly turned to combinatorial approaches that permit the rapid analysis of libraries of protein variants. Phage-displayhasprovedtobeapowerful tool foranalyzingprotein stabilitydue to the large library sizeand the robustnessof the phageparticle toavarietyofdenaturingconditions.

7p dell39 03-04-2013 33 3   Download

Intracellularly expressed antibodies (intrabodies) have been used to inhibit the function of various kinds of protein inside cells. However, problems with stability and functional expression of intrabodies in the cytosol remain unsolved. In this study, we show that single-chain variable fragment (scFv) intrabodies constructed with a heavy chain variable (VH) leader signal sequence at the N-terminus were translocated from the endoplasmic reti-culum into the cytosol of T lymphocytes and inhibited the function of the target molecule, Wiskott–Aldrich syndrome protein (WASP)....

14p dell39 27-03-2013 36 3   Download

While many antibodies with strong antigen-binding affinity have stable variable regions with a strong antibody heavy chain variable region frag-ment (VH)⁄antibody light chain variable region fragment (VL) interaction, the anti-lysozyme IgG HyHEL-10 has a fairly strong affinity, yet a very weak VH⁄VLinteraction strength, in the absence of antigen.

11p inspiron33 26-03-2013 39 4   Download

It has long been an important task to prepare a catalytic antibody capable of digesting a targeting crucial protein that controls specific life functions. Tumor necrosis factor-a(TNF-a) is a cytokine and an important molecule concerned with autoimmune diseases such as rheumatoid arthritis, chronic obstructive pulmonary disease, and Crohn’s disease.

10p viettel02 19-02-2013 34 2   Download

The antigen-binding site of immunoglobulins is formed by six regions, three from the light and three from the heavy chain variable domains, which, on association of the two chains, form the conventional antigen-binding site of the antibody.

9p cosis54 04-01-2013 43 4   Download

The plasma cell disorders are monoclonal neoplasms related to each other by virtue of their development from common progenitors in the B lymphocyte lineage. Multiple myeloma, Waldenström's macroglobulinemia, primary amyloidosis (Chap. 324), and the heavy chain diseases comprise this group and may be designated by a variety of synonyms such as monoclonal gammopathies, paraproteinemias, plasma cell dyscrasias, and dysproteinemias.

22p ozon_ozon 26-04-2012 75 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Regeneration of the immunoglobulin heavy-chain repertoire after transient B-cell depletion with an anti-CD20 antibody...

11p thulanh12 13-10-2011 32 1   Download