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HeLa cell
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We present an assay to experimentally test the regulatory effects of genetic variants within transcripts using CRISPR/ Cas9 followed by targeted sequencing. We applied the assay to 32 premature stop-gained variants across the genome and in two Mendelian disease genes, 33 putative causal variants of eQTLs, and 62 control variants in HEK293T cells, replicating a subset of variants in HeLa cells.
10p
vibransone
28-03-2024
7
2
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Quercetin, a multifunctional therapeutic agent, is used in various types of cancer. However, its therapeutic effect is limited by virtue of poorly aqueous solubility and instability in the physiological medium. To overcome these limitations, we aimed (i) to design quercetin loaded liposomes with unlinked-PEG4000 with regard to not only surface modification but also solubility enhancement, and (ii) to investigate the antineoplastic effects on HeLa cells.
13p
lyhuyenthu
31-01-2023
2
2
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The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a-e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a-e). We examined the effect of the structural difference of complexes on anticancer activity via different arenes and metal centers. Antiproliferative activity of 5a-h and 3a was tested against human cervix adenocarcinoma (HeLa) and rat glioblastoma (C6) cell lines by ELISA assay.
26p
lyhuyenthu
31-01-2023
4
2
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Despite great progress in complex gene regulatory mechanisms in the dynamic tumor microenvironment, the potential contribution of long noncoding RNAs (lncRNAs) to cancer cell metabolism is poorly understood. Death receptor 5 antisense (DR5-AS) is a cisplatin inducible lncRNA whose knockdown modulates cell morphology. However, its effect on cell metabolism is unknown. The aim of this study is to examine metabolic changes modulated by cisplatin and DR5-AS lncRNA in HeLa cells.
14p
lyhuyenthu
31-01-2023
9
2
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Marine fungi are evaluated as a potential source for new natural compounds with bioactivities of pharmaceutical values. In this study, 66 fungal strains, isolated from 37 sponge samples in Nha Trang bay, were determined for cytotoxic activity against two human cancer cell lines, including cervical cancer (Hela) and breast cancer (MCF-7) cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.
7p
vipagani
24-10-2022
4
2
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Sarang semut (Myrmecodia pendans) or ant-nest plant is a potential medicinal plant. The water extract of this plant is wellknown to cure many kinds of disease including cancer. Phytochemicals present in this plant are suspected to be responsible for its health benefits.
4p
vichristinelagarde
04-07-2022
10
1
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This study aimed to develop a comprehensive quantification of 20 bioactive compounds in Achillea millefolium L (A. millefolium) fractions obtained by different solvents (petroleum ether (pet-ether), ethyl acetate (EtOAc), methanol (MeOH) and water) using HPLC quantitative analysis.
7p
schindler
30-12-2021
9
0
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The antimicrobial activity of the products was evaluated and the results revealed that compounds 8f and 15f showed strong activity against gram-positive bacteria while compound 15d showed the highest activity against gramnegative bacteria. Moreover, compounds 15b, 8d, 8e, 8c, 8l, and 8j exhibited significant antifungal activity. In addition, the antitumoral activity of the synthesized products against different cancer cell lines was determined and the results revealed that compound 12c was the most active against MCF-7, HepG-2, HCT-116, and HeLa with IC50 values of 0.51, 0.72, 0.95, and 0.
22p
langthannam
29-12-2021
14
0
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Thiazolylpyrazoline derivatives were recently reported as potent anticancer agents. In this study, novel 1,4-phenylene-bis-N-thiocarbamoylpyrazoles (3a–h) and 1,4-phenylene-bis-pyrazolylthiazoles (5a–h) were prepared and screened for their anticancer activities against C6 (rat brain tumor cells) and HeLa (human uterus carcinoma). Anticancer activity studies were performed as a dose-dependent assay starting with eight concentrations. 5-Fluorouracil (5-FU) was used as a positive control.
11p
langthannam
29-12-2021
10
0
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Investigation of the aerial parts of Cephalaria elazigensis var. purpurea afforded one new oleanane-type saponin, namely cephoside A (1), and five known natural compounds (2–6). Compound 2, named anemoclemoside A, which is an unusual triterpene glycoside, was identified in the family Dipsacaceae for the first time. Chemical structures of all compounds were determined on the basis of the HRESIMS and 1D and 2D NMR data. Cephoside A (1) and anemoclemoside A (2) were assessed for their cytotoxic activities against HeLa cells, having IC50 values of 495 and 135 µg/mL, respectively.
9p
langthannam
29-12-2021
2
0
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The aim of this study was to obtain an oil extract from a Bulgarian green microalga strain Coelastrella sp. BGV and to assess its anticancer and apoptogenic activity in vitro against human tumor cells HeLa by means of MTT and fluorescence microscopy analyses. The results showed that the oil extract obtained by a direct extraction using a common vegetable oil reduces proliferation and induces apoptosis in HeLa cells.
3p
mudbound
10-12-2021
16
1
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Therefore, in this study, we examined the biological and pharmaceutical properties, focusing on cell-targeted toxicity, organism-targeted toxicity (zebrafish) and anticancer activity on cervical cancer in vitro, of the ethanol extract of the medicinal plant C. kongensis (CK-EE), an endemic plant of Vietnam used as traditional medicine for a long period.
7p
guitaracoustic02
08-12-2021
18
2
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As part of the ongoing efforts to exploit the pharmaceutical potential of domestic cyanobacteria, six strains belonging to the Nostocales order have been isolated from several sampling sites in Hanoi as prerequisite material. The cytotoxic activity evaluation based on the MTT test resulted in four extracts of two strains (HK7 and NK1111) exhibiting the inhibitory activity against HeLa cells, with IC50 values ranging from 84.6 µg/mL to 257.3 µg/mL.
11p
guitaracoustic02
08-12-2021
8
1
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Novel 1-substituted 3-(p-isopropylphenyl)-5-phenylformazans (3a–g) were synthesized and characterized by elemental analysis, 1H NMR, and FT-IR techniques and UV-visible spectroscopy. Antiproliferative activities of 3a–g against HeLa and C6 cells were determined using the BrdU cell proliferation ELISA assay. 5-Florouracil was used as the positive control. The effects of substituents (–H, –CH3, and –I) and their positions (ortho, meta, and para) on the antiproliferative activities were evaluated.
9p
tudichquannguyet
29-11-2021
8
1
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In this research, a new series of (E) -3-(4-substitutedphenyl)-1-(4’-morpholinophenyl)prop-2-en-1-one derivatives 1–7 was synthesized, aiming to develop effective antiproliferative agents. The antiproliferative activities of compounds 1–7 were examined against HeLa and C6 cell lines at eight different concentrations using the BrdU ELISA assay. The activity results were compared with reference anticancer drug 5-fluorouracil (5-FU). Compound 1 had almost the same antiproliferative activity as 5-FU.
19p
tudichquannguyet
29-11-2021
6
0
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A total of 63 newly synthesized compounds were screened in terms of PARP inhibition by cellular PARylation assay in the HeLa cell line. It was found that 19 compounds significantly inhibited the H2O2 -induced cellular PARylation. The chemosensitizer effect of these compounds in cancer cells treated with doxorubicin (doxo) was investigated. It was found that the combination of potent PARP inhibitors with doxo potentiated a cytotoxic effect, similar to that of olaparib.
26p
tudichquannguyet
29-11-2021
6
0
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Chemical investigation of the lichen Parmotrema praesorediosum (Nyl.) Hale led to isolate six phenolic compounds including praesorediosic (1), orcinol (2), orselinic acid (3), lecanorin (4), isolecanoric acid (5) and virensic acid (6). Among them, compound 1 appeared to be found for the first time in the nature. The structure of these compounds was elucidated by spectroscopic analyses of HRESIMS and NMR as well as the comparison of their NMR data with those in the literature.
5p
nguaconbaynhay11
16-04-2021
20
1
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Their structures were established by means of physical data (ESI MS, 1D and 2D NMR) and evaluated for the cytotoxic activities against liver hepatocellular carcinoma (HepG2), human lung cancer (NCI-H460), human epithelial carcinoma (HeLa) and human breast cancer (MCF-7) cell lines. This is the first report on phytochemical studies of this plant.
5p
nguaconbaynhay11
16-04-2021
19
2
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In this study, we conducted the experiments to extract flavonoids from tea leaves and perform initially in vitro tests about the effects of obtained extracts with two cancer cell lines: uterine and breast cancer.
7p
kethamoi9
01-12-2020
13
2
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A phytochemical investigation of whole Aspidistra letreae plants led to the isolation of 2Hchromen-2-one (1), α-tocopherol (2), (E)-phytol (3), asparenydiol (4) and (25S)-spirost-1β,3α,5β-triol (5). Their structures were determined on the basis of NMR spectral evidences and in comparison with the reported data. Of these, asparenydiol (4) was isolated from the genus Aspidistra for the first time. This is also the first report on the separation and structural determination of (25S)-spirost-1β,3α,5β-triol (5) as a pure compound.
9p
nguaconbaynhay9
03-12-2020
11
2
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