Heparin chromatography

The purification of extracellular vesicles (EVs) remains a major hurdle in the progression of fundamental research and the commercial application of EV-based products. In this study, we evaluated the potential of heparin affinity chromatography (HAC) to purify neural stem cell-derived EVs as part of a multistep process.

16p viginny 23-12-2022 6 3   Download

Fibroblast growth factor-2 (FGF-2) is a multifunctional protein that plays an important role in the regulation of proliferation, differentiation and migration of a variety of cells. The recombinant human FGF-2 (rhFGF-2) is currently used in stem cell culture, medicine and cosmetic products. In this study, we aim to produce secreted rhFGF-2 protein from a Pichia pastoris strain containing multiple copies of the fgf-2 gene to eliminate the disadvantages of intracellular expression systems

9p larachdumlanat127 02-01-2021 12 2   Download

Pseudoviruses (PsVs) that encapsidate a reporter plasmid DNA have been used as surrogates for native human papillomavirus (HPV), whose continuous production is technically difficult. HPV PsVs have been designed to form capsids made up of the major capsid protein L1 and the minor capsid proteins L2.

10p vihamax2711 21-04-2020 8 0   Download

For the analysis of biological samples two blood samples were taken, one with 5 ml heparin for the determination of acetyl cholinesterase (AChE) and pseudo cholinesterase (PChE) by the technique of Michel and Aldrige (Vorhaus and Kark, 1953) and another of 5 ml without anticoagulant for the analysis of OC pesticides in serum.

442p conquynho32 18-09-2012 77 9   Download