Homozygous doubled haploid

In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research tools in genome studies as well as in studies of commercially important traits.

10p vihagrid 30-01-2023 7 3   Download

Anther culture is an effective breeding method for improving desirable traits, since it allows fast achievement of genetically homozygous lines. The present studies were carried out to evaluate the response of flue-cured tobacco anthers for high frequency pollen embryo induction in different culture media, and the effects of colchicine treatment on tobacco chromosome doubling. The results showed that different media caused significant changes in pollen embryo induction, in which the most obvious change was found in B medium with induction rates of 23.28%, 26.64%, and 28.

9p tudichquannguyet 29-11-2021 5 1   Download

Sporophytic plants having gametophytic chromosome number are termed as haploid. These plants can be produced either spontaneously in nature or by in vitro or in vivo induction technique. By doubling chromosome number of haploid either spontaneously or artificially, double haploid (DH) could be produced. Double haploid are homozygous and this homozygosity is achieved in one generation by using DH production systems. This production system is used for fixation of heterosis. Genetic map construction is relatively easy with DH population.

15p nguaconbaynhay5 16-05-2020 7 1   Download

Conventional breeding involves time taking procedure for pyramiding of genes and screening of crops for the same. Double haploid (DH) production can reduce the time of production of resistant crops in less time period. Twenty one plants that survived after the hardening procedure were screened phenotypically and molecularly for BB resistant genes.

8p nguaconbaynhay3 07-02-2020 14 0   Download

A doubled haploid (DH) is a genotype developed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding. Double haploid (DH) breeding can helps in speedup conventional plant breeding programmes and make early release of cultivars with superior and desirable traits along with greater utility in other research aspects of plant breeding, genetics and genetic engineering. DH’s helps in complementing back cross breeding by transferring genes of interest from wild relatives thus breaking genetic barriers.

18p nguaconbaynhay3 07-02-2020 20 0   Download

The combined effect of anther incubation time on CP induction medium (12, 14, and 16 days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the effectiveness of androgenesis was investigated in 17 genotypes of Capsicum grown in Poland.

7p sony2711 01-02-2019 28 1   Download

To create new varieties resistant to biotic and abiotic stress factors that possess high productivity and improved fruit quality, breeders require modern breeding methods and techniques. Double haploid (DH) lines are an invaluable breeding element due to their guaranteed, completely homozygous nature.

8p sony2711 28-01-2019 17 3   Download