Human cell lines

Though the hybridoma technology has been widely applied in the production of monoclonal antibodies, it has existed some disadvantages including low yield and genetic instability. Therefore, an alternative approach should be taken into account. Recently, recombinant monoclonal antibody technology has emerged as the best choice to cure hybridoma related drawbacks.

10p vibego 02-02-2024 3 1   Download

Human epidermal growth factor receptor 2 (HER2) has been identified as a molecular target for breast cancer therapy, such as Trastuzumab (Herceptin®). This has been shown to improve patient survival substantially.

10p vibego 02-02-2024 7 0   Download

This study investigated the in vitro effects of ZnO NP and chemical UV filters commonly used sunscreens on the function of immortalised and primary skin cells. ZnO particulates (30 nm, 80 nm and 200 nm) were compared to the chemical UV filters: butylmethoxydibenzoylmethane (BMDBM) ethylhexylmethoxycinnamate (EHMC), ethylhexylsalicylate (EHS), homomenthylsalicylate (HMS) and 4-methylbenzylidenecamphor (MBC). Human epidermal keratinocytes (NHEK), epidermal melanocytes (NHEM), dermal fibroblasts (NHDF) and the immortalised keratinocyte HaCaT cell line were used in this study.

324p runthenight05 01-03-2023 9 3   Download

Recent sequencing technologies enable joint quantification of promoters and their enhancer regions, allowing inference of enhancer–promoter links. We show that current enhancer–promoter inference methods produce a high rate of false positive links. We introduce FOCS, a new inference method, and by benchmarking against ChIAPET, HiChIP, and eQTL data show that it results in lower false discovery rates and at the same time higher inference power.

14p vimichaelfaraday 25-03-2022 16 2   Download

Demonstrating the main chemical constituents of Anodendron paniculatum (Roxb.) A.DC. Structural determination of isolated compounds; evaluation of the cytotoxic activities of isolated compounds against human cancer cell lines.

25p nguathienthan12 23-05-2021 13 3   Download

There is extensive evidence that FcR c-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR c-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR c-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin....

10p system191 01-06-2013 35 3   Download

Selenoprotein P (SeP) is a plasma protein that contains up to 10 selenocysteine residues and accounts for about 50% of total selenium in human plasma. We have previously shown that SeP expression in the human liver cell line HepG2 is inhibited by transforming growth factor (TGF)-b1 on a transcriptional level. Smad proteins are the transcriptional mediators of TGF-b signalling and putative Smad-binding elements (SBE) comprising the core sequence CAGACA are present at two positions in the SeP promoter....

6p system191 01-06-2013 35 4   Download

Connective tissue growth factor/hypertrophic chondrocyte specific gene product 24 (CTGF/Hcs24) promotes proliferation and differentiation of chondrocytes in culture. We investigated the roles of two major types of mitogen activated protein kinase (MAPK) in the promotion of proliferation and differentiation by CTGF/Hcs24. Here we report the effects of the MAPKK/MEK 1/2 inhibitor, PD098059, and p38 MAPK inhibitor, SB203580, in a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) and rabbit growth cartilage (RGC) cells treated with CTGF/ Hcs24....

8p system191 01-06-2013 40 3   Download

The human MUC4 gene encodes a large membrane-associated mucin, characterized by a mucin tandem repeat domain and a growth factor-like transmembrane domain. In addition to the originally published sequence (sv0-MUC4), several MUC4 cDNA sequences (called sv1-MUC4 to sv21MUC4, MUC4/X, MUC4/Y) from various tissues and cell lines have been recently described. They differ from sv0MUC4 by deletions and/or insertions located in the 3¢ region or, for two of them, by deletion of the central repetitive domain.

8p research12 01-06-2013 42 4   Download

Subunits (a, b and c) of the interleukin-2 receptor complex (IL-2R) are involved in both proliferative and activationinduced cell death (AICD) signaling of T cells. In addition, the signaling b and c chains are shared by other cytokines (e.g. IL-7, IL-9, IL-15). However, the molecular mechanisms responsible for recruiting/sorting the a chains to the signaling chains at the cell surface are not clear.

10p research12 01-06-2013 29 5   Download

The spermine analogue N1,N11-diethylnorspermine (DENSPM) efficiently depletes the cellular pools of putrescine, spermidine and spermine by down-regulating the activity of the polyamine biosynthetic enzymes and up-regulating the activity of the catabolic enzyme spermidine/ spermine N1-acetyltransferase (SSAT). In the breast cancer cell line L56Br-C1, treatment with 10 lM DENSPM induced SSAT activity 60 and 240-fold at 24 and 48 h after seeding, respectively, which resulted in polyamine depletion. ...

7p research12 01-06-2013 53 5   Download

Matrilysin (matrix metalloproteinase-7) plays important roles in tumor progression. It was previously found that matrilysin binds to the surface of colon cancer cells to promote their metastatic potential. In this study, we identified annexin II as a novel membrane-bound substrate of matrilysin. Treatment of human colon cancer cell lines with active matrilysin released a 35 kDa annexin II form, which lacked its N-terminal region, into the culture supernatant. The release of the 35 kDa annexin II by matrilysin was significantly enhanced in the presence of serotonin or heparin....

14p research12 29-04-2013 39 2   Download

Emerin is a ubiquitously expressed inner nuclear membrane protein of unknown function. Mutations in its gene give rise to X-linked Emery–Drei-fuss muscular dystrophy (X-EDMD), a neuromuscular condition with an associated life-threatening cardiomyopathy. We have previously reported that emerin is phosphorylated in a cell cycle-dependent manner in human lymphoblastoid cell lines [Ellis et al. (1998) Aberrant intracellular targeting and cell cycle-dependent phosphorylation of emerin contribute to the EDMD phenotype.J. Cell Sci. 111, 781–792]. ...

14p research12 29-04-2013 41 4   Download

Hexadecylphosphocholine (HePC) is a synthetic lipid rep-resentative of a new group of antiproliferative agents, alkylphosphocholines (APC), which are promising candi-dates in anticancer therapy. Thus we have studied the action of HePC on the human hepatoblastoma cell line HepG2, which is frequently used as a model for studies into hepatic lipid metabolism.

7p research12 23-04-2013 42 1   Download

We have analyzed the response of a number of human cell lines to treatment with antisense oligodeoxynucleotides (ODNs) directed against RNA polymerase II, replication protein A, and Ha-ras. ODN-delivery to the cells was liposome-mediated or via electroporation, which resulted in di€erent intracellular locations of the ODNs. The ODN-mediated target mRNA reduction varied consider-ably between the cell lines.

10p research12 23-04-2013 38 3   Download

The present study was conducted on human Jurkat T-cell lines in order to elucidate the role of phospholipase A2in capacitative calcium entry. We have employed thapsigar-gin (TG)that induces increases in [Ca 2+ ] i by emptying the calcium pool of endoplasmic reticulum, followed by capacitative calcium entry. We designed a Ca 2+ free/Ca 2+ reintroduction (CFCR)protocol for the experiments, conducted in Ca 2+ -free medium.

7p tumor12 22-04-2013 30 2   Download

Cystatin F is a cysteine peptidase inhibitor recently discov-ered in haematopoietic cells by cDNA cloning. To further investigate the expression,distribution and properties of the native human inhibitor the promyeloid cell line U937 has beenstudied.The cells expressedrelatively largequantities of cystatin F,which was found both secreted and intracellu-larly. The intracellular levels were unusually high for a secreted cystatin (
25% of the cystatin F in 2- or 4-day culture medium).

10p tumor12 22-04-2013 46 2   Download

The structure–activity relations of a series of synthetic phenoxazone drugs with aminoalkyl side chains of variable length and different terminal groups were investigated by examining their biological activity and DNA complexation affinity. Biological activitywas determined fromtheir ability to induce apoptosis and cell cycle perturbations (activation of cell cycle checkpoints) using the human malignant MOLT-3 cell line. The thermodynamic parameters of drug– DNA complexation were determined by differential scan-ning calorimetry....

8p tumor12 20-04-2013 37 4   Download

Peptides which should be generated from the neuropeptide FF (NPFF) precursor were identified in a neuronal (human neuroblastoma SH-SY5Y) cell line and in COS-7 cells after transient transfection of the human proNPFFAcDNA and were comparedwith those detected in themouse spinal cord. After reverse-phase high performance liquid chromatogra-phy of soluble material, NPFF-related peptides were im-munodetected with antisera raised against NPFF and identified by using on-line capillary liquid chromatography/ nanospray ion trap tandem mass spectrometry. ...

13p tumor12 20-04-2013 32 2   Download

The heat-stable enterotoxin peptides (ST) produced by enterotoxigenicEscherichia coliare one of the major causes of transitory diarrhea in the developing world. Toxin bind-ing to its receptor, guanylyl cyclase C (GC-C), results in receptor activation and the production of high intracellular levels of cGMP. GC-C is expressed in two differentially glycosylated forms in intestinal epithelial cells. Prolonged exposure of human colonic cell lines to ST peptides induces cellular refractoriness to the ST peptide, in terms of intra-cellular cGMP accumulation....

10p tumor12 20-04-2013 37 3   Download