Human sperm

To inititally evaluate the efficiency of cryoprotectant-free vitrification in PCR tubes on a low number of human spermatozoa. Methods: A laboratory experimental study was conducted on 30 severe oligospermia treated at the Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University.

8p vijaychest 16-05-2024 2 1   Download

Ebook "Male germline stem cells: Developmental and regenerative potential" covers many of the fundamental issues now being explored by today’s generation of stem cell researchers, including the field’s potential for regenerative medicine. Ranging from an assessment of the pluripotency of primordial germ cells and their possible applications in treating testicular cancer, to the recovery of once-mordant fertilization-competent sperm, this volume has it all.

269p ladongphongthanh1008 22-04-2024 4 1   Download

Ebook "Sperm chromatin: Biological and clinical applications in male infertility and assisted reproduction" covers the fundamental principles of sperm chromatin architecture and function, the proposed modes of DNA damage and repair, the tests of sperm DNA damage, the clinical aspects of DNA damage and the impact of DNA damage on reproductive outcome.

515p tracanhphuonghoa1007 22-04-2024 3 2   Download

Part 2 book "Chromatography - The most versatile method of chemical analysis" by Leonardo de Azevedo Calderon includes contents: Chromatographic analysis of nitrogen utilization and transport in arbuscular mycorrhizal fungal symbiosis; instrumental analysis of tetrodotoxin; chromatography as the major tool in the identification and the structure function relationship study of amylolytic enzymes from saccharomycopsis fibuligera R64; isolation and purification of sperm immobilizing agglutinating factors from bacteria and their corresponding receptors from human spermatozoa,...

198p muasambanhan09 16-03-2024 5 1   Download

Part 2 book "Animal models and human reproduction" includes content: The mare as an animal model for reproductive aging in the woman, spotlight on reproduction in domestic dogs as a model for human reproduction, animal models of inflammation during pregnancy, prezygotic chromosomal examination of mouse spermatozoa, molecular and cellular aspects of mammalian sperm acrosomal exocytosis,... and other contents.

350p muasambanhan04 07-01-2024 4 2   Download

In this study, we will summarize the results of several studies on the harmful effects of prolonged high-dose use of paracetamol on sperm number, quality, portability and testicular morphology in experimental animals and humans.

15p vispacex 16-11-2023 3 2   Download

We examined the properties of the nanocomposite γ-Fe2O3@Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV–vis absorption.

9p vihermione 06-01-2023 7 2   Download

During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis.

14p vidarwin 23-02-2022 9 1   Download

This study included 22 women in intracytoplasmic sperm injection cycle. The expression of olfactory target molecules in cumulus cells surrounding the growing and mature oocytes was evaluated by Western blotting and real-time polymerase chain reaction. Additionally, integrated bioinformatics analyses were carried out and 6 ectopic olfactory receptors were selected for further evaluation. The initiation of olfactory transduction cascade in cumulus cells of competent oocytes was confirmed by analyzing the expression of adenylyl cyclase type 3 and olfactory market protein.

14p thiencuuchu 27-11-2021 15 1   Download

The relationship between aging and epigenetic profiles has been highlighted in many recent studies. Models using somatic cell methylomes to predict age have been successfully constructed. However, gamete aging is quite distinct and as such age prediction using sperm methylomes is ineffective with current techniques.

10p vitzuyu2711 29-09-2021 13 1   Download

Semen cryopreservation is a widely used technique across the world for conservation and proliferation of genetically superior germplasm through artificial insemination. Artificial insemination (AI) is the most conclusive tool to increase genetic improvement, control of venereal diseases and improve herd performance and productivity. For achieving the mission, successful and effective cryopreservation of semen is essential.

18p nguaconbaynhay1 04-12-2019 33 0   Download

Numerous studies have identified specific body systems affected by the hazardous effects of the cigarette smoking particularly the respiratory and cardiovascular systems. The effect of smoking on male reproduction has also been studied where semen quality was investigated in different cross-sectional studies including infertile patients with conflicting results. This article aimed to assess the relationship between smoking and male infertility.

8p kethamoi1 20-11-2019 14 1   Download

This study assessed the association between fructose and zinc concentration and various seminal characteristics. Fructose and zinc in semen reflect the secretory function of seminal vesicles. These tests may help in assessing the diagnosis and the management of male infertility

10p cumeo4000 01-08-2018 40 2   Download

Binding of lamin B receptor (LBR) to chromatin was studied by means of an in vitro assay system involving recombinant fragments of human LBR and Xenopus sperm chromatin. Glutathione-S-transferase (GST)-fused proteins including LBR fragments containing the N-terminal region (residues 1–53) and arginine-serine repeat-containing region (residues 54–89) bound to chromatin. The binding of GST-fusion proteins incorporating the N-terminal and arginine-serine repeat-containing regions to chromatin was suppressed by mild trypsinization of the chromatin and by pretreatment with a DNA solution....

11p research12 01-06-2013 27 3   Download

The FDA also has jurisdiction over screening and testing of reproductive tissues, such as the eggs and sperm that will be implanted in human recipients. Regulations issued by the agency contain strict requirements for egg and sperm donors, including thorough medical histories, identification controls, freedom from infectious diseases, and rigorous inspection of the facilities in which these tissues are handled. Inspectors can order the recall or destruction of tissue that is infected with a communicable disease.

28p le_minh_nha 25-12-2012 42 3   Download

This book contains four parts with 16 chapters. Firstly, an optimal stimulation scheme  for  ovaries,  particularly  natural  and  minimal  stimulation  of  ovaries,  has  been  discussed  in  the  first  part.  Then,  one  paper  analyzed  that  how  many  oocytes  per  retrieval will be  the best  for human IVF practice. If one stimulation scheme produces  too  many  eggs,  it  often  results  in  hyperstimulation  syndrome.  Thus,  a  chapter  reviewed  hyperstimulation  syndrome  diagnosis,  prevention  and  treatment.

148p yeutinh98 22-09-2012 63 8   Download

The past three decades have witnessed significant advances in the field of assisted human conception. Following the remarkable perseverance and triumph of Robert Edwards, Patrick Steptoe, and Jean Purdy, numerous scientists and physicians from around the world have worked to develop more effective and safer procedures to treat infertile couples.

459p socolanong 23-04-2012 105 12   Download

Human spermatozoa at ejaculation are incapable of in vivo fertilization and must undergo maturational change during which they acquire the ability to fertilize oocytes. This process, known as capacitation, was described more than 50 year ago by both Austin (1) and Chang (2). Capacitation is prevented in ejaculated spermatozoa by at least one factor in seminal plasma (3). Additionally, prolonged exposure to seminal plasma can inhibit the ability of spermatozoa to undergo the acrosome reaction in vitro (4) and diminish their capacity to fertilize (5).

14p lananhanh123 27-08-2011 56 5   Download

Transmission of Genetic Disease Origins and Types of Mutations A mutation can be defined as any change in the primary nucleotide sequence of DNA regardless of its functional consequences. Some mutations may be lethal, others are less deleterious, and some may confer an evolutionary advantage. Mutations can occur in the germline (sperm or oocytes); these can be transmitted to progeny. Alternatively, mutations can occur during embryogenesis or in somatic tissues.

5p konheokonmummim 03-12-2010 69 3   Download