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Hybrid embryos
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Ebook "Immunocytochemical methods and protocols (Second edition)" are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
447p
coduathanh1122
27-03-2024
3
1
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The agricultural pest Ceratitis capitata, also known as the Mediterranean fruit fly or Medfly, is a fruit crop pest of very high economic relevance in different continents. The strategy to separate Ceratitis males from females (sexing) in mass rearing facilities is a useful step before the sterilization and release of male-only flies in Sterile Insect Technique control programs (SIT).
10p
vinarcissa
21-03-2023
3
1
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In conclusion, activation by cycloheximide was the most qualified chemical for artificial activation and preimplantation development of the Bos gaurus-taurus interspecies embryo. However, H3K9me3 immunostaining can only detect the Bos gaurus pronucleus formation at the zygote stage.
8p
vimalfoy
08-02-2023
5
1
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Growing oocytes derived from small antral follicles (2-3 mm) have poor maturation rates and developmental competence compared to fully grown oocytes collected from large antral follicles (4-6 mm). Hence, a pre-in vitro maturation (Pre-IVM) subculture with in vitro maturation (IVM) was applied to support small oocytes growth.
8p
vimalfoy
08-02-2023
3
1
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Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox17 and Cxcr4 are useful markers of the DE. Previously, we identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos.
9p
viwyoming2711
16-12-2020
14
1
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Animals with polyploid, hybrid nuclei offer a challenge for models of gene expression and regulation during embryogenesis. To understand how such organisms proceed through development, we examined the timing and prevalence of mortality among embryos of unisexual salamanders in the genus Ambystoma.
15p
viwyoming2711
16-12-2020
17
1
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Embryo rescue is an in vitro technique that has been used to save the hybrid products of fertilization when they might otherwise degenerate. Embryo culture applied to practical problems is a tissue culture technique that has proven to be of greatest value to breeders. One of the main uses of this technique is to produce interspecific and intergeneric hybrids. Post-zygotic barriers such as endosperm abortion and embryo degeneration leading to low fertility have been overcome through the use of embryo culture and several hybrids have been developed in horticultural crops.
9p
nguaconbaynhay5
11-05-2020
6
1
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Successful interspecific hybrids were obtained when we crossed the cultivated soybean with wild annual Glycine soja. Although the success rate was low, whereas, the success rate was zero in case of wild perennial G. tomentella. Probably there is need to improve our crossability skills. Alternately, embryo rescue technique may be employed to recover the hybrids between cultivated soybean and wild perennial species. The hybridity between cultivated and wild annual soybean (Glycine soja) hybrids was confirmed at morphological and molecular level.
13p
cothumenhmong3
22-02-2020
13
0
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Berseem, a nitrogen-fixing, annual, multicut forage crop cultivated around 2 million hectares areas of northern, central and eastern parts of India. Berseem has variability for pollination behavior however variation for morphological and agronomic traits are scare, perhaps, because of initially introduction of crop with narrow genetic base. Genetic variability have been fortified through introduction of exotic materials, intra-interspecific hybridization, induction of polyploidy and mutation. ICAR-Indian Grassland and Fodder Research Institute maintain >900 accessions of Trifolium spp.
9p
nguaconbaynhay1
04-12-2019
22
0
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Somatic embryogenesis was initiated from immature zygotic embryos of Abies cilicica Carr. and its hybrid A. cilicica x A. nordmanniana. Schenk and Hildebrandt medium (SH) supplemented with 5 µM benzylaminopurine was used as the initiation medium.
6p
viminotaur2711
30-10-2019
14
0
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Rose breeders need reliable and efficient germination protocols to exploit all viable embryos in their breeding programs. In this study, different treatment combinations were assessed to overcome the mechanical resistance of the pericarp and enhance germination of rose seeds obtained from hybridisation among hybrids roses.
9p
vi4mua
24-02-2019
14
1
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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Micropropagation of hybrid walnut trees (Juglans nigra x Juglans regia) through culture and multiplication of embryos...
4p
toshiba9
12-10-2011
40
3
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