Hybridomas

Bài giảng Nhập môn Công nghệ sinh học: Chương 5 Công nghệ sinh học động vật, cung cấp cho người học những kiến thức như: Nuôi cấy tế bào động vật; Hybridoma và các kháng thể đơn dòng; Nhân bản vô tính ở động vật có vú; Nuôi cấy tế bào gốc. Mời các bạn cùng tham khảo!

22p caphesuadathemhanh 03-12-2021 34 5   Download

Retinoids are potent immune modulators that inhibit Fas ligand (FasL) expression and thereby repress the activationinduced apoptosis of immature thymocytes and T-cell hybridomas. In this study, we demonstrate that all-transretinoic acid (all-trans-RA) directly represses the transcriptional activity of the nuclear factors of activated T-cells (NFAT), which is an important transactivator of the FasL promoter.

9p research12 01-06-2013 43 5   Download

Hybridomas are cells that have been engineered to produce a desired antibody in large amounts. To produce monoclonal antibodies, Bcells are removed from the spleen of an animal that has been challenged with the relevant antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is performed by making the cell membranes more permeable. The fused hybrid cells (called hybridomas), being cancer cells, will multiply rapidly and indefinitely and will produce large amounts of the desired antibodies.

7p zingzing09 24-04-2013 71 4   Download

Four mAbs of the IgG1 class to the thrombin-treated N-terminal disulfide knot of fibrin, secreted by various hybridomas, have been selected. Epi-topes for two mAbs, I-3C and III-10d, were situated in human fibrin frag-ment Bb15–26, and those for two other mAbs, I-5G and I-3B, were in fragment Bb26–36.

10p media19 04-03-2013 23 1   Download