Hydrolytic cellulases

Genomic studies demonstrate that components of virulence mechanisms in filamentous eukaryotic pathogens (FEPs, fungi and oomycetes) of plants are often highly conserved, or found in gene families that include secreted hydrolytic enzymes (e.g., cellulases and proteases) and secondary metabolites (e.g., toxins), central to the pathogenicity process.

15p viansan2711 30-07-2021 10 1   Download

Polysaccharide monooxygenases (PMOs) catalyze oxidative degradation of recalcitrant carbohydrate chains in cellulose, starch, and chitin. In biofuel industry, the conversion of rich lignocellulose source to fermentable sugars by hydrolytic cellulases can be synergistically boosted by cellulose-active PMOs (AA9 PMOs) found in a vast number of fungi that grow on biomass.

6p vimariana2711 21-12-2020 12 0   Download

Specific activity of two hydrolytic enzymes viz., cellulase and polygalactouranase was assayed in the shattering and non-shattering zones of pod wall and lignin content in pod wall of soybean varieties at four harvesting stages viz., at physiological maturity, 5 DAPM, 10 DAPM and 15 DAPM. The cellulase enzyme activities was lowest at non-shattering zone of pod wall of the susceptible varieties as compare to the resistant varieties but highest at shattering zone of pod wall of the susceptible varieties indicating its role in pod shattering.

12p gaocaolon5 14-06-2020 8 0   Download

The present study was focused on the biodegradation of pine needles by the production of hydrolytic enzymes viz. cellulase, xylanase, laccase as well as reducing sugars production w.r.t optimization of moisture (Basal salt media) ratio for the newly isolated white rot fungi Phanerochaete sp. S7, Armillaria sp. K3 and Phanerochaete chrysosporium [NCIM 1197] were optimized under solid state fermentation and maximum of cellulase (52.27 U/g) with specific activity of 2.10 was observed in P.

14p chauchaungayxua3 07-02-2020 12 1   Download

A cellulase [endo-b-1,4-D-glucanase (EC 3.2.1.4)] was iso-lated from the hepatopancreas of abalone Haliotis discus hannaiby successive chromatographies on TOYOPEARL CM-650M, hydroxyapatite and Sephacryl S-200 HR. The molecular mass of the cellulase was estimated to be 66 000 Da by SDS/PAGE, thus the enzyme was named HdEG66. The hydrolytic activity of HdEG66 toward carb-oxymethylcellulose showed optimal temperature and pH at 38
Cand 6.3, respectively.

8p tumor12 20-04-2013 43 3   Download