Hydrophobic interaction chromatography
-
Differential scanning calorimetry was established for in-situ measurement ofthe transition temperatures of antibodies when adsorbed on hydrophobic interaction chromatography media. This method is also suitable for non-transparent media, which is an advantage over spectroscopic methods that cannot be used in many cases due to large background signals.
7p viginny 23-12-2022 5 3 Download
-
The adsorption behavior of the model proteins: alpha-Lactalbumin, Bovine Serum Albumin, Lysozyme, and a monoclonal antibody, in single component and in binary mixtures, was investigated on two different hydrophobic interaction chromatography resins using both static and dynamic methods.
12p viginny 23-12-2022 10 3 Download
-
Deploying two salts in hydrophobic interaction chromatography can significantly increase dynamic binding capacities. Nevertheless, the mechanistic understanding of this phenomenon is lacking. Here, we investigate whether surface tension or ionic strength govern dynamic binding capacities of the chromatographic resin Toyopearl Butyl-650 M in dual salt systems.
12p viginny 23-12-2022 6 3 Download
-
Screening of physicochemical properties should be considered one of the essential steps in the drug discovery pipeline. Among the available methods, biomimetic chromatography with an immobilized artificial membrane is a powerful tool for simulating interactions between a molecule and a biological membrane.
8p viginny 23-12-2022 6 2 Download
-
In the present study, both of these challenges were addressed through the development of an innovative mixed mode resin with 2-amino-4methylpentanoic acid ligands that combines weak cation exchange with moderate hydrophobic interactions.
11p vironald 15-12-2022 7 2 Download
-
Size exclusion chromatography has become an essential tool for the protein therapeutics industry. Conceptually, it is a simple form of chromatography that is driven by entropy and sieving effects. An ideal size exclusion column would exhibit no adsorptive interactions between its internal surfaces and the solutes being analysed, but that is not easily achieved.
10p vironald 15-12-2022 7 2 Download
-
A carboxymethylcellulose (CMC)-degrading bacterium was isolated from soil, identified as Bacillus methylotrophicus according to the physiological properties and analyses of 16S rRNA and a partial sequence of the gyrase A (gyrA) gene, and named as B. methylotrophicus Y37. The CMCase enzyme was purified to homogeneity by 20.4-fold with 21.73% recovery using single-step hydrophobic interaction chromatography and biochemically characterized. CMCase showed a molecular weight of approximately 50 kDa as determined by SDS-PAGE.
14p langthannam 29-12-2021 8 0 Download
-
The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics.
8p vihamax2711 21-04-2020 12 0 Download
-
Intestinal triglyceride lipase (TG) of Cyprinion macrostomus after precipitation with 30% polyethylene glycol (PEG), was partially purified by hydrophobic interaction chromatography on phenyl sepharose CL-4B, and the effect of pH on this enzyme activity was determined titrimetrically. Intestinal tissue homogenates were precipitated using 30% PEG-8000, and then applied onto a phenyl sepharose CL-4B column for hy-drophobic interaction chromatography.
11p vitamix 19-02-2019 25 0 Download
-
After studying this chapter you will be able to: Know that primary structure determines protein conformation, define domains, motifs combine through hydrophobic interactions to form domains, know general properties of a protein with 4o structure, know the definition of an oligomeric protein, know 4o structure nomenclature, be able to determine the subunit composition of an oligomeric protein using information obtained from gel permeation chromatography and SDS PAGE analysis,...
16p tangtuy02 08-03-2016 68 3 Download
-
We purified an extracellular pyranose dehydrogenase (PDH) from the basidiomycete fungusAgaricus xanthodermausing ammonium sulfate frac-tionation and ion-exchange and hydrophobic interaction chromatography. The native enzyme is a monomeric glycoprotein (5% carbohydrate) con-taining a covalently bound FAD as its prosthetic group.
16p inspiron33 23-03-2013 34 3 Download
-
Protein oligomeric complexes have emerged as a major target of current research because of their key role in aggregation processes in living systems andin vitro. Hydrophobic and charged surfaces may favour the self-assembly process by recruiting proteins and modifying their interactions. We found that equine lysozyme assembles into multimeric complexes with oleic acid (ELOA) at the solid–liquid interface within an ion-exchange chromatography column preconditioned with oleic acid.
15p vinaphone15 25-02-2013 46 2 Download