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Identification and antimicrobial resistance
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Part 1 book "Textbook of microbiology and immunology" includes content: History of microbiology; morphology and physiology of bacteria; sterilization and disinfection; culture media; culture methods; laboratory identification of bacteria and taxonomy; bacterial genetics; genetic engineering and molecular methods; antimicrobial agents - therapy and resistance,... and other contents.
320p
muasambanhan10
11-04-2024
7
1
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Klebsiella spp. are opportunistic pathogens which can cause severe infections, are often multi-drug resistant and are a common cause of hospital-acquired infections. Multiple new Klebsiella species have recently been described, yet their clinical impact and antibiotic resistance profiles are largely unknown.
16p
vibransone
28-03-2024
1
1
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Part 1 book "Salmonella - Methods and protocols" includes content: Luminex® multiplex bead suspension arrays for the detection and serotyping of salmonella spp.; quantitative proteomic identification of host factors involved in the salmonella typhimurium infection cycle; determination of antimicrobial resistance in salmonella spp.; red mediated recombineering of salmonella enterica genomes; a method to introduce an internal tag sequence into a salmonella chromosomal gene,... and other contents.
129p
muasambanhan01
13-12-2023
9
4
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This study was aimed to investigate the intestinal microbiota in racing pigeons with regard to Enterococcus species distribution, virulence factors and antibiotic susceptibility. Three methods (API, Multiplex sodA-PCR, 16S rRNA sequencing) were compared for Enterococcus species identification. Cloacal samples from 179 apparently healthy pigeons of 13 different flocks were tested.
14p
vigalileogalilei
28-02-2022
22
1
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Salmonella enterica serovars are a major cause of foodborne illness and have a substantial impact on global human health. In Canada, Salmonella is commonly found on swine farms and the increasing concern about drug use and antimicrobial resistance associated with Salmonella has promoted research into alternative control methods, including selecting for pig genotypes associated with resistance to Salmonella.
12p
vigalileogalilei
28-02-2022
20
1
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Staphylococcus pseudintermedius (SP) and Staphylococcus aureus (SA) are common colonizers of companion animals, but they are also considered opportunistic pathogens, causing diseases of diverse severity. This study focused on the identification and characterization of 33 coagulase-positive staphylococci isolated from diseased pets (28 dogs and five cats) during 2009–2011 in a veterinary hospital in Spain in order to stablish the circulating lineages and their antimicrobial resistance profile.
10p
vidarwin
23-02-2022
11
1
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We describe a method that adds long-read sequencing to a mix of technologies used to assemble a highly complex cattle rumen microbial community, and provide a comparison to short read-based methods. Long-read alignments and Hi-C linkage between contigs support the identification of 188 novel virus-host associations and the determination of phage life cycle states in the rumen microbial community.
18p
vielonmusk
30-01-2022
15
0
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Klebsiella is an opportunistic bacterial pathogen of man and animals known for presence of diversity of Anti Microbial Resistance (AMR). The present study was undertaken to isolate and study the anti-microbial resistance in Klebsiella isolates from cattle and buffaloes. Sixty (60) samples, comprising of 33 mastitic milk and 27 diarrhoeic faecal samples were collected aseptically from cattle and buffaloes. From these 60 samples, 11 (18.33%) Klebsiella species were isolated and identified on the basis of cultural, morphological and biochemical characteristics.
5p
caygaocaolon11
21-04-2021
10
2
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Blood stream infections are very common in paediatric age group and are one of the common causes of morbidity and mortality. Septicaemia gradually leads to serious consequences like shock, multi organ failure, DIC etc. Timely identification of causative pathogen by blood culture (gold standard) is important. Antibiogram also helps us to elevate consciousness on resistance problems and recognise prospect to decrease the use of inappropriate antibiotics. The study was undertaken to evaluate major bacterial isolates causing septicaemia and their antibiogram pattern.
5p
cothumenhmong9
18-01-2021
13
2
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Acinetobacter baumannii has emerged as a highly troublesome pathogen for many institutions globally. As a consequence of its immense ability to acquire or upregulate antibiotic drug resistance determinants, it has justifiably been propelled to the forefront of scientific attention. The clinical specimens over a period of 3 years (January 2014 to December 2016) were analyzed and the A. baumannii isolates obtained by an automated identification system (Vitek 2 Compact) were segregated for further study.
6p
cothumenhmong9
18-01-2021
14
3
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The study was done in Department of Microbiology, RMC, Loni. The clinical samples were subjected to Gram staining, aerobic culture using MacConkey and blood agar. Acinetobacter species were identified by standard microbiological identification methods. Antimicrobial susceptibility testing was done by Kirby Bauer disc diffusion technique and according to CLSI guidelines.
9p
angicungduoc8
07-11-2020
12
1
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The study was aimed to determine the prevalence and drug resistance pattern of P. aeruginosa in SSI. Over a 2-year period, specimens were received as postoperative wound swabs in Microbiology department and processed for isolation and identification of bacterial pathogens, according to the standard microbiological techniques.
5p
trinhthamhodang6
09-07-2020
15
2
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Escherichia coli (E. coli) is one of most common organisms responsible for health careassociated urinary tract infection (HAUTI). The aim of this study was to determine antibiotic susceptibility pattern of E. coli isolated from HAUT I and to study ciprofloxacin resistance caused by gyrA gene mutations among these isolates. Urine Samples were collected from patients with suspected HAUTI. E. coli identification and antimicrobial susceptibility testing, minimum inhibitory concentration (MIC) of ciprofloxacin were done followed by detection of mutations in the gyrA gene by PCR-RFLP.
6p
nguaconbaynhay6
24-06-2020
10
0
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The present study was conducted on various clinical samples, in the Department of Microbiology, S.N medical college, Agra from February 2018 to July 2018. 72 Enterococcus isolates were identified. Maximum samples from which Enterococcus spp. was isolated is urine i.e. 39 (54.17%), followed by pus and blood i.e. 22 (30.55%) and 5 (6.94%) respectively. Maximum isolate is E. faecalis i.e. 62 (86.11%) followed by E.faecium i.e. 10 (13.89%). On Antimicrobial Susceptibility testing, Ampicillin, Ciprofloxacin, Levofloxacin, Tetracycline and Doxycycline show 70.83%, 38.88%, 30.55%, 58.33% and 52.
9p
gaocaolon5
14-06-2020
10
1
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Blood stream infections cause a significant morbidity and mortality worldwide. Rapid and reliable detection of bacterial pathogens and rational use of antimicrobial are required for proper management. The Aims of this study includes Isolation and identification of pathogens from blood samples using automated BacT/ALERT blood culture system and their antibiotic resistance patterns.
8p
angicungduoc5
12-06-2020
14
1
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The aim of this study is to analyze probiotic properties of isolated lactic acid bacteria from human breast milk. Identification of lactic acid bacterial (LAB) was done by Gram’s staining and catalase test and further confirmation was based on morphological, cultural, physiological and different biochemical tests. The isolated strain was identified after different biochemical analysis which was also showed reliable probiotic properties. These isolates were examined for further probiotic properties including tolerance to bile salt and resistance to low PH, antimicrobial activity.
10p
angicungduoc5
12-06-2020
13
0
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Staphylococcus aureus is an important nosocomial pathogen responsible for mortality and morbidity in nearly most of the patients admitted in tertiary care centre because of its ability to induce methicillin resistance and its ability to show resistance to macrolides, lincosamides, type B streptogramin type and other groups of antibiotics resulting in treatment failure. MLSB strains whose expression can be constitutive (CMLSB) or inducible (iMLSB) is encoded by ermA and ermC genes.
7p
caygaocaolon5
27-05-2020
11
1
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To isolate common organisms from suspected cases of septicemia with their antimicrobial susceptibility pattern and know the prevalence of MDR strains by detecting MRSA isolates, ESBL, AmpC and Carbapenemase production. Blood samples were collected from suspected cases of septicemia and incubated in BacT/ALERT 3D. Subculture was done on Blood agar, nutrient Agar, MacConkey Agar and Chocolate Agar and identification was done by standard biochemical reactions.
11p
trinhthamhodang1212
06-04-2020
8
1
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Pyoderma is the commonest skin infection in children. The awareness about regional pattern of isolates, their antibiotic sensitivity and resistance pattern is of utmost importance in treating them. Aims of the study are 1) To study the bacteriological profile of pyoderma in children 2) To assess the antibiotic sensitivity pattern. 3) To determine the resistance pattern. Cases of primary and secondary pyoderma in paediatric age group attending Dermatology outpatient department in a tertiary care teaching hospital over a period of one year were enrolled in this study.
8p
trinhthamhodang1212
06-04-2020
47
3
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Diabetic foot infections (DFIs) is a chronic form of diabetes mellitus (DM) associated with a high economic and social problem worldwide. Approximately 60% of these amputations is preceded by the presence of infected ulcers. Recently, Enterococci, mainly Entrococcus feacalis (E. faecalis), is considered one of the most frequent microorganisms isolated from hospital associated infections in in many parts of the world.
19p
trinhthamhodang4
22-03-2020
12
1
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