Identification by conventional

A proteomic workflow for a simple loss-less manual nano-fractionation (300 nL/fraction) for low μg sample amounts which avoids the need to dry down or transfer fractions to autosampler vials is shown to be feasible. It is demonstrated that the conventional procedure of drying samples down followed by reconstitution negatively affects the number of protein and peptide identifications.

10p viginny 23-12-2022 8 2   Download

Surgical cricothyroidotomy is a last resort in patients with an anticipated difficult airway, but without any guarantee of success. Identification of the cricothyroid membrane may be the key to successful cricothyrotomy. Ultrasonographic identification of the cricothyroid membrane has been reported to be more useful than the conventional palpation technique. However, ultrasonographic identification techniques are not yet fully characterized.

4p vizhangzhidong 29-12-2021 10 0   Download

Crustacean hyperglycaemic hormone (CHH) is a neuropeptide that was originally identified in the X-organ/sinus gland complex of the eyestalks (ESs) in crustaceans. Several CHH isoforms and spliced variants were later identified in other tissues, and their functions have still not been completely unveiled. In this study, the identification and characterisation of the conventional CHH prepropeptide from the ESs of the littoral crab, Carcinus aestuarii, via rapid amplification of cDNA ends was reported.

10p dolomite36 30-12-2021 7 0   Download

The conventional variant calling of pathogenic alleles in exome and genome sequencing requires the presence of the non-pathogenic alleles as genome references. This hinders the correct identification of variants with minor and/or pathogenic reference alleles warranting additional approaches for variant calling.

8p vibeauty 23-10-2021 10 1   Download

The banana (Musa sp., AAA) genome is constantly increasing due to high-frequency of somaclonal variations. Due to its large diversity, a conventional numerical and morphological based taxonomic identification of banana cultivars is laborious, difficult and often leads to subject of disagreements.

11p vijeeni2711 30-06-2021 12 1   Download

Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function.

5p vimaine2711 26-03-2021 14 2   Download

Candida is an opportunistic fungi responsible for causing Candidiasis when the patients immunity is low. Emergence of Nonalbicans Candida along with Candida albicans is also increasing. Increased use of antifungal drugs, use of antibiotics, long term use of catheters has contributes to resistance to antifungal drugs. Isolation of Candida species by conventional and Hichrome agar was done. Antifungal sensitivity testing was done by disc diffusion method.

7p chauchaungayxua11 23-03-2021 11 1   Download

In this paper, the authors propose a novel adaptive multilayer T-S fuzzy controller (AMTFC) with a optimize soft computing algorithm for a class of robust control uncertain nonlinear SISO systems. First, a new multilayer T-S fuzzy was created by combined multiple simple T-S fuzzy model with a sum function in the output. The multi-layer fuzzy model used in nonlinear identification has many advantages over conventional fuzzy models, but it cannot be created by the writer's experience or the trial and error method. It can only be created using an optimization algorithm.

13p trinhthamhodang9 04-12-2020 15 2   Download

This study included the isolation of Klebsiella pneumoniae bacteria from different sources in the environment and identification by using API-20E system and a conventional Polymerase chain reaction assay which detects Klebsiella pneumoniae wbbZ gene that involved in expression of D-galacton. The results of our study confirmed the positive identification of the bacteria using API-20E analytical system and also the presence of wbbZ gene in two of Klebsiella pneumoniae tissue invasive strains using conventional PCR assay.

8p nguathienthan8 20-10-2020 13 1   Download

The parent PT 6129 showed higher total carotenoids (8.99 µg/g) and β carotene content (1.71 µg/g) than the other parent and it is having yellow grain colour, while, the parent PT 6029 recorded grey colour grain with low β carotene content (0.61 µg/g) which indicated that yellow colour contributed to β carotene content. The progenies, TNBG-06-53-1-4-7 recorded high total carotenoids (8.10 µg/g) and β carotene (1.73 µg/g) followed by TNBG-06-81-10-9-9, TNBG-06-127-3-3-1, TNBG-06-81-4-8-6 and TNBG06-3-3-6-1.

9p trinhthamhodang7 31-08-2020 10 1   Download

Rotavator is an efficient tillage implement used for rapid bed preparation and is an energy and time efficient equipment for different soils compared to all other conventional tillage implements. The primary cause that limits the persistence of rotavator is wear of its blades. This paper was undertaken to study the material composition and wear characteristics of austempered ductile iron third edition rotavator blades which were developed by austempering heat process done over cast iron.

12p caygaocaolon6 30-07-2020 20 2   Download

Mycoflora population from soil of organic and conventional fields was investigated at different plots. The fungi were isolated by using soil dilution method. Total of six genera were obtained from 10 soil samples. On the basis of cultural and microscopic characteristics, the isolated strains were identified as Aspergillus sp. Penicillium sp., Alternaria sp., Fusarium sp., Cladosporium sp. and Gliocladium sp. Aspergillus species showed the highest percentage among the six genera.

13p trinhthamhodang6 09-07-2020 14 1   Download

This study was conducted on one hundred and fifty clinical isolates of S. aureus isolates forming three groups (group one fifty community acquired isolates from adult infections, group two: fifty community acquired isolates from pediatric infections and group three:fifty hospital acquired isolates as a control group) isolated from different clinical specimens. All isolates were identified by conventional methods such as morphological identification, gram stain, and catalase test. Identification to species level by slide and tube coagulase, culture on DNase agar and mannitol salt agar

9p nguaconbaynhay6 23-06-2020 8 1   Download

The present study was conducted to study the prevalence of the food borne zoonotic pathogen of animal origin, L. monocytogenes by isolation and identification, molecular detection and antibiotic sensitivity pattern from different samples of cattle sources in Aizawl district of Mizoram. A total 200 numbers of sample including cattle faeces (50), raw milk (50) and milk products (100) were collected randomly from different unorganized shop and farms.

15p gaocaolon5 14-06-2020 11 2   Download

Oxidase positive, non-fermenting, Gram negative bacilli are invariably incorrectly identified as Pseudomonas species in conventional culture methods often missing out isolates of Ochrobactrum anthropi. We report here the clinical and microbiological characteristics of this rare pathogen from a case of neonatal sepsis. Rapid isolation and correct identification followed by appropriate antimicrobial therapy was the key to favourable clinical outcome.

4p angicungduoc4 26-04-2020 19 2   Download

The present study was done to find rapid and accurate identification of bacterial species which will help in prevention and cure of diseases in various aquatic animals. Phenotypic identification of bacterial isolates was done by conventional tests and commercial methods. Antibiotic sensitivity study revealed only gatifloxacin and chloramphenicol were found to be effective, both inhibited 83.33% sensitivity to the bacterial strains. Prevalence study revealed that aeromoniais were the dominant disease.

14p trinhthamhodang4 22-03-2020 9 1   Download

Neonatal sepsis is one of the significant reasons for morbidity and mortality among the neonates in the developing countries. To determine the bacterial isolates and their antibiotic susceptibility pattern. Blood cultures were performed for all clinically suspected neonatal septicemia cases for 1 year. Identification of all pathogenic isolates was done by conventional methods followed by antibiotic sensitivity testing by Kirby bauer disk diffusion method. A total of 225 suspected for neonatal septicemia were included in this study. Among this 78(34.

6p nguathienthan3 27-02-2020 26 0   Download

E. coli is a Gram negative bacteria belonging to enterobacteriacea family. They are commonly found in the intestine of animals and humans. Most E. coli are harmless but some can cause infection. In our study, we isolated E.coli from 100 (50 from sheep and 50 from goats) faecal samples of diarrhoeic as well as healthy sheep. Isolation was done on BHI, MLA and EMB agar. The isolated bacteria suspected for E. coli were subjected to biochemical tests viz catalase, oxidase and IMViC. The isolates confirmed by biochemical tests were further confirmed by genus specific PCR.

7p cothumenhmong3 22-02-2020 17 0   Download

Enteric fever is a global health problem and is also endemic in India. The etiologic agents of enteric fever i.e. S. typhi and S. paratyphi occurs mostly through food-borne or waterborne transmission. Worldwide, however, there are an estimated 22 million cases of enteric fever annually, with 600,000 deaths and an estimate of the annual typhoid incidence rate of 493.5 per 100,000 person years was reported from India in 2008. The laboratory diagnosis of enteric fever is very important mainly in this post-antibiotic era.

8p trinhthamhodang3 12-02-2020 19 0   Download

NFGNB also known as Non-fermenters (NFs) are emerging with increasing frequency as agents of opportunistic and often, serious infection as well as nosocomial infection. NFGNB are innately resistant to many antibiotics and are known to produce extended spectrum β-lactamases and metallo β-lactamases (MBL).Very few laboratories in India identify these organisms as routine as they are slow growing and requires special media and biochemical tests for identification. The rates of isolation of NFGNBs are increasing in Clinical Microbiology Laboratory, hence this study was done.

10p nguaconbaynhay3 07-02-2020 24 1   Download