Immunofluorescence assay

Cytokines play an essential role in the pathogenesis related to the severity, monitor progress, and evaluate ICS therapeutic response in asthma. Research objectives: Evaluate changes in serum levels of IL4, IL5, IL13, and TNFα in patients with asthma control according to GINA after 3 months.

9p viengfa 28-10-2024 2 1   Download

Baculoviruses are well known for their large, circular, dou-ble-stranded DNA genomes. The type member, AcMNPV, is the best characterized andundergoes a successionof early, late and very late gene expression during its infection cycle. The viral genes involved inDNAreplicationhave previously been identified and their products are required for the acti-vation of late gene expression.

8p tumor12 22-04-2013 50 3   Download

The transcription factor p63 plays a pivotal role in the development and maintenance of epithelial tissues, including the ocular surface. In an effort to gain insight into the pathogenesis of keratitis caused by HSV-1, we determined the expression patterns of the p63 and Bax proteins in the Staatens Seruminstitute Rabbit Cornea cell line (SIRC). Methods: SIRC cells were infected with HSV-1 at various multiplicities and maintained for different periods of time. Virus replication was measured by indirect immunofluorescence assay and Western blot analysis.

8p toshiba23 18-11-2011 39 2   Download

Migration of vascular smooth muscle cells (SMCs) from the media to intima constitutes a critical step in the development of proliferative vascular diseases. To elucidate the regulatory mechanism of vacular SMC motility, the roles of caldesmon (CaD) and its phosphorylation were investigated. Methods: We have performed Transwell migration assays, immunofluorescence microscopy, traction microscopy and cell rounding assays using A7r5 cells transfected with EGFP (control), EGFP-wtCaD or phosphomimetic CaD mutants, including EGFP-A1A2 (the two PAK sites Ser452...

12p toshiba23 18-11-2011 45 4   Download