Immunohistochemical staining method

To investigate the role of NudCD1 in spindle assembly checkpoint regulation and in the prognosis of colorectal cancer. Methods: Immunohistochemical staining was used to detect in situ expression of NudCD1 in 100 colorectal cancer tissue samples. A chi-square test was used to analyse the correlation between the NudCD1 protein expression level of the cancer tissues and clinicopathological features.

13p vialfrednobel 23-12-2023 3 3   Download

Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors with a high prevalence and poor prognosis. It is an urgent problem to deeply understand the molecular mechanism of ESCC and develop effective diagnostic and prognostic methods.

9p vioracle 29-09-2023 4 2   Download

There is a growing interest in the scientific community to use computer-based software programs for the quantification of cells during physiological and pathophysiological processes. Drawbacks of computer-based methods currently used to quantify immunohistochemical staining are the complexity of use, expense of software and overly-simplified descriptions of protocol thereby limiting reproducibility.

8p vigandhi 23-02-2022 9 1   Download

In this study, we investigated CD20+ TILs in triple-negative breast cancer (TNBC) and their relationship with T lymphocyte subsets (CD4+, CD8+, CD25+, and FOXP3+), including their combined prognostic value using an immunohistochemical staining method.

10p vianrose2711 27-04-2021 14 2   Download

To investigate on expressions and clinical significances of CD133 protein and vasculogenic mimicry (VM) in primary non-small cell lung cancer (NSCLC). Methods: The specimens of NSCLC from 305 Chinese patients with follow-up were analyzed for CD133 protein expression and VM by immunohistochemical and histochemical staining.

9p vijisoo2711 30-09-2020 7 1   Download

The purpose of this study is to evaluate the prognostic value of TFPI-2 expression in breast cancer patients through examining the correlation between TFPI-2 expression and breast cancer clinicopathologic features. Methods: Immunohistochemical staining combined with digital image analysis was used to quantify the expression of TFPI-2 protein in breast tumor tissues.

9p vijisoo2711 29-09-2020 13 1   Download

This study was carried out to evaluate the prognostic value of KIBRA in breast cancer. Methods: This retrospective study included breast cancer patients who sought the services of the immunohistochemistry laboratory of our unit from 2006 to 2015. Tissue microarrays were constructed and immunohistochemical staining was done to assess the KIBRA expression.

11p vibaku2711 22-07-2020 14 1   Download

Primary enteric-type adenocarcinoma of the urinary bladder is relatively uncommon. We present our experience with 109 pure, non-urachal cases—the largest series to date. This work was undertaken with the aim of describing the immunohistochemical features of adenocarcinoma of the urinary bladder associated with schistosomiasis, illustrating their histologic and immunohistochemical similarities to colorectal carcinomas.

6p kethamoi1 20-11-2019 22 1   Download

Objectives: To determine some histopathological features and evaluate the expression of some IHC markers in prostate carcinoma (Pca). Subjects and methods: Histopathological study was performed on 84 specimens by using hematoxylin and eosin (H.E) stained tissue sections. IHC study was performed on 33 specimens by using IHC stained tissue sections with PSA, CK34βE12, p63, CK7, CK5/6, actin monoclonal antibodies.

6p jangni3 20-04-2018 20 1   Download

The goals of experimental neuroscience research are fundamentally to gain mechanistic understanding of the pathology of disease in order to identify appropriate targets for potential pharmacological intervention and evaluation of putative therapies. With the advances in gene manipulation and transgene technologies, we have unprecedented ability to generate animal models of disease that more closely mimic the clinical conditions. Noninvasive techniques capable of investigating altered pathophysiologies are now of paramount importance.

366p echbuon 02-11-2012 59 5   Download