In situ hybridization
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Evaluation of human epidermal growth factor receptor 2 (HER2) overexpression caused by erbb2 receptor tyrosine kinase 2 (ERBB2) amplification (AMP) by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) is essential for treating unresectable metastatic gastric cancer (GC).
13p vikoch 27-06-2024 1 1 Download
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This article, we present a thorough and straightforward hydrothermal method towards the production of reduced graphene oxide templated MIL-53(Fe) hybrids RGO@MIL-53(Fe). The structural characteristics of created hybrids were identified by using BET, FESEM, powder X-ray diffraction, and FTIR techniques. Methylene blue (MB) dye (a model water pollutant) was used to evaluate H2O2-assisted photo-catalytic efficiency of RGO@MIL-53(Fe).
9p dianmotminh02 03-05-2024 1 1 Download
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Ebook "Microfluidic methods for molecular biology" covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis.
382p tracanhphuonghoa1007 22-04-2024 2 1 Download
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Ebook "Immunocytochemical methods and protocols (Second edition)" are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
447p coduathanh1122 27-03-2024 3 1 Download
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Ebook "Alien introgression in wheat: Cytogenetics, molecular biology, and genomics" provides an overview of the latest advancements in the field of alien introgression in wheat. The discovery and wide application of molecular genetic techniques including molecular markers, in situ hybridization, and genomics has led to a surge in interspecific and intergeneric hybridization in recent decades. The work begins with the taxonomy of cereals, especially of those species which are potential gene sources for wheat improvement.
387p manmanthanhla0201 26-02-2024 4 1 Download
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Part 1 book "Veterinary infection biology molecular diagnostics and high throughput strategies" includes content: Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections; identification of animal pasteurellaceae by maldi tof mass spectrometry; gold nanoparticles as a potential tool for diagnosis of fish diseases; nucleic acid testing, new platforms and nanotechnology for point of decision diagnosis of animal pathogens,... and other contents.
328p muasambanhan02 18-12-2023 4 0 Download
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This study aimed to report the prevalence of HER2-neu in newly diagnosed early or metastatic gastric cancer (GC) patients, to determine the percentage of patients achieving various IHC scores correlating with the ISH results and to establish a database for GC patients in Lebanon.
8p vileonardodavinci 23-12-2023 5 3 Download
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HER2 expression has a prognostic and predictive impact in early-stage breast cancer (BC). HER2 positive BC (immunohistochemistry (IHC) score 3+or 2+with in situ hybridization (ISH) amplifcation) are treated with HER2 targeted therapies.
10p vileonardodavinci 23-12-2023 10 4 Download
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Long noncoding RNAs (lncRNAs) are abnormally expressed in a broad type of cancers and play significant roles that regulate tumor development and metastasis. However, the pathological roles of lncRNAs in esophageal squamous cell carcinoma (ESCC) remain largely unknown. Here we aimed to investigate the role and regulatory mechanism of the novel lncRNA RPL34-AS1 in the development and progression of ESCC.
13p vialfrednobel 23-12-2023 3 3 Download
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Large-scale genomic alterations, especially CD274/PD-L1 gene amplification, have great impact on anti-PD-1 efficacy on cancers such as Hodgkin’s lymphoma. However, the prevalence of PD-L1 genetic alterations in colorectal cancer (CRC) and its correlation with the tumor immune microenvironment and clinical implications remain unknown.
11p vischultz 20-10-2023 1 1 Download
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ADP-ribosylation factor-like protein 4 C (ARL4C) is a member of the ARF small GTP-binding protein subfamily. The ARL4C gene is highly expressed in colorectal cancer (CRC). ARL4C protein promotes cell motility, invasion, and proliferation.
6p visharma 20-10-2023 4 2 Download
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Low human epidermal growth factor receptor 2 (HER2) expression is an emerging concept in breast cancer that is defined as immunohistochemistry (IHC) 1+or IHC 2+and negative in situ hybridization (ISH) but has been poorly investigated. The aim of our study was to determine the frequency of low HER2 expression among HER2- negative breast cancers and compare the clinicopathological features and prognosis of HER2-low patients with those of HER2-zero patients.
8p vioracle 29-09-2023 2 2 Download
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Benzene is a chemical widely used in industrial settings and known to have hematopoietic toxicity. Previous literature has been inconsistent regarding a direct association of benzene exposure with lymphoma.
3p viintuit 26-09-2023 1 0 Download
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Myxoid liposarcoma is the second most common type of liposarcoma that typically presents in the lower extremities and has a predilection for men. Manifestation of this tumor in the lower back is rare. We report the first known case of an adult woman presenting with a soft tissue mass in her lower back.
3p viintuit 26-09-2023 3 0 Download
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The Leguminosae family is the third-largest family of angiosperms, and Caesalpinioideae is its secondlargest subfamily. A great number of species (approximately 205) are found in the Caesalpinia group within this subfamily; together with these species’ phenotypic plasticity and the similarities in their morphological descriptors, make this a complex group for taxonomic and phylogenetic studies.
10p vinarcissa 21-03-2023 2 1 Download
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Cultivated barley belongs to the tertiary genepool of hexaploid wheat. Genes of interest can be transferred from barley into wheat through wide hybridization. The application of wheat-barley introgression lines could provide an excellent tool for the transfer of earliness, favourable amino acid composition, biotic stress resistance, abiotic stress tolerance, or good tillering ability into wheat.
13p vinarcissa 21-03-2023 4 1 Download
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Although Tellinidae is one of the largest and most diverse families of bivalves, its taxonomy is utterly chaotic. This is mainly due to the morphological diversity and homoplasy displayed by their shells and to the scarcity of the molecular phylogenetic studies performed on them. A molecular cytogenetic analysis of four tellin shell species, Bosemprella incarnata, Macomangulus tenuis, Moerella donacina and Serratina serrata, was performed.
7p vinarcissa 21-03-2023 2 1 Download
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The ability to eliminate a parental genome from a eukaryotic germ cell is a phenomenon observed mostly in hybrid organisms displaying an alternative propagation to sexual reproduction. For most taxa, the underlying cellular pathways and timing of the elimination process is only poorly understood.
9p vinarcissa 21-03-2023 3 1 Download
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The Bactrocera dorsalis species complex currently harbors approximately 90 different members. The species complex has undergone many revisions in the past decades, and there is still an ongoing debate about the species limits.
10p vinarcissa 21-03-2023 3 1 Download
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Mussels of the genus Mytilus present morphologically similar karyotypes that are presumably conserved. The absence of chromosome painting probes in bivalves makes difficult verifying this hypothesis. In this context, we comparatively mapped ribosomal RNA and histone gene families on the chromosomes of Mytilus edulis, M. galloprovincialis, M. trossulus and M. californianus by fluorescent in situ hybridization (FISH).
11p vinarcissa 21-03-2023 3 1 Download