In vitro root formation

This study investigated the effects of different PGRs on in vitro plant regeneration of Anthurium scherzerianum Schott and acclimatization in the ex vitro stage. Depending on the objective of each experiment, the in vitro shoots were induced from callus cultured on a modified Murashige and Skoog (MS) medium containing cytokinins and auxins.

11p vicaptainmarvel 21-04-2023 3 2   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p capheviahe27 23-02-2021 19 4   Download

In this study, the role of IBA (indole3-butyric acid), method of placing node on root induction were investigated. The results showed that SH (Schenk & Hildebrandt) medium supplemented with 2 mg/L IBA was suitable for adventitious root induction of Morinda officinalis cultured in vitro.

8p kequaidan8 02-11-2020 9 1   Download

The shoots in vitro were transferred to half-strength MS supplemented with different concentrations of IAA, IBA and NAA to investigate root formation. The best rooting occurred at 0,5 mg/L NAA (7.91 roots/shoot; root length of 4.01 cm; 98.51% root formation). The plantlets with uniform growth were planted on different substrate: Eco clean soil, Coconut fiber, Fern fiber, 50% Rice husk in combination with 50% Eco clean soil for research the most suitable substrate.

9p nguyenxuankha_bevandan 14-08-2020 13 1   Download

Rhizoctonia root rot of okra caused by Rhizcotonia solani is an important disease which is known to cause heavy loss in Rajasthan as well as in India. Among physiological parameters effect of temperature, effect of pH and effect of relative humidity were study in vitro. The fungus grew at all the temperature level ranged from 15ºC to 35ºC. Maximum mycelial growth of the fungus (90mm) was observed at 30ºC. In different pH levels that maximum dry mycelium weight (630gm) of Rhizoctonia solani was observed at 6.5 pH level.

8p caygaocaolon6 30-07-2020 7 1   Download

Root rot of soybean is an important disease of soybean inflicting heavy losses in crop. The present investigation was carried out to test the efficacy of agrochemicals and fertilizers in vitro. In bioassay test of non systemic fungicides, Zineb 75 WP gave cent per cent inhibition of growth and sclerotial formation at minimum concentration of 500 ppm followed by copper hydroxide 77WP (53.63%).

8p angicungduoc6 22-07-2020 4 1   Download

The effects of different culture media and temperature on mycelial growth and sclerotial formation of Macrophomina phaseolina (Tassi) Goid inciting agent of castor root rot were studied in vitro. Eight different solid and liquid media were tested for their effect on mycelial growth and sclerotial formation of test fungus. Among the solid media, potato dextrose agar (90.00 mm) and Richard's agar (87.71 cm) were the best for fungus growth as well as for sclerotial formation followed by Elliott's medium (80.08 cm).

5p cothumenhmong4 25-03-2020 16 1   Download

Papaya is one of the few important fruits which flowers and yield throughout the year. Though a polygamous species with three basic types viz., staminate, pistillate and bisexual, its cultivation with seed propagation is hindered by problems due to vide variability in sex expression and fruit characters. In such point of view, propagation through asexual method could be a better alternative. The micropropagation will substitute method of propagation by cutting or grafting would be of great boon to papaya industry. The procedure for in vitro propagation of papaya (Carica papaya) cv.

6p quenchua2 15-12-2019 17 0   Download

Arbuscular Mycorrhizal Fungi benefits plants by improving the uptake of phosphate and other nutrients from soil and also increases the disease tolerance to the host plant. The fungal pathogens cause huge loss to vegetable crops when infected. In order to overcome this problem, the influence of AMF in the control of fungal plant pathogens is studied. AMF, Glomus sp. was isolated from the rhizosphere soil of tomato plants. The wilt pathogen, Fusarium oxysporum f.sp. lycopersici was cultured from the infected roots of tomato plants.

11p kethamoi2 15-12-2019 15 0   Download

The results showed that protocorms were formed from seeds culture on the ½ MS medium supplemented with 10% potatoes extract within 8 weeks. Protocorms which were cultured on the ½ MS medium containing 30 g/l sucrose, 0.5 g/l AC, 7 g/l agar, 0.1 mg/l NAA and 1 mg/l BA were optimal for PLB formation (8.67 PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, 0.5 g/l AC, and 7 g/l agar after about 90 days.

8p slimzslimz 08-12-2019 12 1   Download

Chilli is grown in many states in India as a valuable trade crop. Chilli accounts for 20-30% of total Indian spices exports valuing approx Rs. 400-500 cores. Nine cultural media Potato dextrose agar, Host extract agar (Chilli), Oat meal agar, Carrot agar, Plane agar, Richards agar, Czapeks agar, Elliots agar, Saboroud’s agar were analyzed for in vitro mycelial growth of Sclerotium rolfsii. The cultural characteristics indicated that the excellent growth and sclerotia formation were observed on different semi-synthetic media viz.

6p cothumenhmong1 11-12-2019 12 1   Download

This study was carried out to assess the propagation of Tacca leontopetaloides (Taccaceae) in Vitro using seed embryos. The seeds were cultured on Murashige & Skoog basal media augmented with various concentrations of single or combined growth hormones to obtain a suitable protocol for plantlet regeneration. Seed embryo germination of 57% was obtained, as against 20% for germination under conventional sowing. The best shoot proliferation was obtained using media fortified with 0.1 mg/L 6-benzyl amino purine and 0.

6p bichxuan01643027348 15-08-2019 15 0   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p xacxuoc4321 09-07-2019 30 4   Download

This study is to investigate the in vitro propagation and ex vitro root formation of Gnetum gnemon var. griffithii Markgr (G. gnemon), one of the nutrient-rich forest vegetables. The results showed that MS medium supplemented with 1.5 mg l-1 BAP was the most suitable combination for shoot formation after 90 days of culture. The combination between 1 mg l-1 BAP and 0.5 mg l-1 NAA gave the best in vitro rooting rate (40%) after 60 days culture.

9p shiwo_ding7 05-06-2019 14 0   Download

A protocol for production of virus-free Vitis vinifera using meristem culture was developed. Meristems (0.1-0.2 mm) of V. vinifera infected with grapevine fanleaf virus and grape leafroll associated viruses were excised from 1-yearold growing vines.

12p masterbarista 15-01-2019 30 2   Download