Insect cells

This study presents notes on the nesting behavior of the four Indian wasps, namely, Chalybion bengalense (Dahlbom) (Sphecidae), Trypoxylon petiolatum F. Smith (Crabronidae), Delta esuriens (Fabricius) and Xenorhynchium nitidulum (Fabricius) (both, Vespidae: Eumeninae).

9p viambani 18-06-2024 7 1   Download

In this study, the hn gene coding for the HN antigen of NDV genotype VIII was artificially synthesized, ligated into donor vector pFastBacHT A and integrated into the baculovirus genome for the purpose of HN expression in Spodoptera frugiperda 9 (Sf9) cells. The recombinant HN protein was successfully expressed in Sf9 cells in the SF900 III medium.

8p dianmotminh02 03-05-2024 1 1   Download

Part 2 book "Biotechnology" by author John E. Smith includes content: Plant and forest biotechnology, animal and insect biotechnology, food and beverage biotechnology, biotechnology and medicine, stem cell biotechnology, protection of biotechnological inventions, safety in biotechnology, public perception of biotechnology - genetic engineering – safety, social, moral and ethical considerations, looking to the future.

134p muasambanhan10 06-04-2024 0 0   Download

Ebook "Site-directed insertion of transgenes" aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification.

393p nhanphanguyet 28-01-2024 3 2   Download

Ebook "Yellow biotechnology I: Insect biotechnologie in drug discovery and preclinical research" has been introduced as a synonymous term for insect biotechnology, which can be defined as the use of biotechnology-based methods for the development of insects (or their molecules, cells, organs or associated microorganisms) into products and services for specific applications in medicine, plant protection or industry.

201p nhanphanguyet 28-01-2024 4 2   Download

Ebook "Advanced technologies for managing insect pests" among the highlights of this book is the use of novel insecticides acting on a specific site in an insect group and are compatible with natural enemies and the environment. One of such approaches is based on disrupting the activity of biochemical sites acting on transcription factors such as the Helix-Loop-Helix (bHLH) family, anti juvenile hormone (AJH) agents that target JH biosynthetic enzymes, G-protein coupled receptors (GPCR) and bursicon as a target for insect control.

331p lamquandat 28-12-2023 4 2   Download

Part 1 book "Principles of veterinary parasitology" includes content: Veterinary parasitology - basic concepts; arthropods part 1 - introduction and insects; arthropods part 2 - ticks, mites and ectoparasiticides, protozoa (single-celled parasites).

284p muasambanhan02 18-12-2023 2 2   Download

The spotted-wing Drosophila (Drosophila suzukii) is a widespread invasive pest that causes severe economic damage to fruit crops. The early development of D. suzukii is similar to that of other Drosophilids, but the roles of individual genes must be confirmed experimentally. Cellularization genes coordinate the onset of cell division as soon as the invagination of membranes starts around the nuclei in the syncytial blastoderm.

10p vihagrid 30-01-2023 6 3   Download

Citrus worldwide is threatened by huanglongbing (HLB) and tristeza diseases caused by ‘Candidatus Liberibacter asiaticus’ (CaLas) and Citrus tristeza virus (CTV). Although the pathogens are members of the α-proteobacteria and Closteroviridae, respectively, both are restricted to phloem cells in infected citrus and are transmitted by insect vectors. The response of sweet orange to single infection by either of these two pathogens has been characterized previously by global gene expression analysis.

17p vilarryellison 29-10-2021 6 0   Download

Azadirachtin, one of the most promising botanical insecticides, has been widely used for pest control. Azadirachtin induces apoptosis in insect cell lines, including Sf9, SL-1 and BTI-Tn-5B1–4. Mitochondrial and lysosomal pathways are likely involved in the azadirachtin-induced apoptosis, however, detailed molecular mechanisms remain largely undefined.

11p vibeauty 23-10-2021 5 1   Download

Trypanosoma brucei brucei, the parasite causing Nagana in domestic animals, is closely related to the parasites causing sleeping sickness, but does not infect humans. In addition to its importance as a pathogen, the relative ease of genetic manipulation and an innate capacity for RNAi extend its use as a model organism in cell and infection biology. During its development in its mammalian and insect (tsetse fly) hosts, T. b. brucei passes through several different life-cycle stages.

11p vibeauty 23-10-2021 7 1   Download

The immunoglobulin (Ig) superfamily receptor Down syndrome cell adhesion molecule (Dscam) gene can generate tens of thousands of isoforms via alternative splicing, which is essential for both nervous and immune systems in insects. However, further information is required to develop a comprehensive view of Dscam diversification across the broad spectrum of Chelicerata clades, a basal branch of arthropods and the second largest group of terrestrial animals.

14p vibeauty 23-10-2021 4 0   Download

The lower Dipteran fungus fly, Sciara coprophila, has many unique biological features that challenge the rule of genome DNA constancy. For example, Sciara undergoes paternal chromosome elimination and maternal X chromosome nondisjunction during spermatogenesis, paternal X elimination during embryogenesis, intrachromosomal DNA amplification of DNA puff loci during larval development, and germline-limited chromosome elimination from all somatic cells.

23p vitzuyu2711 29-09-2021 12 1   Download

Resistance of pest insect species to insecticides, including B. thuringiensis (Bt) pesticidal proteins expressed by transgenic plants, is a threat to global food security. Despite the western corn rootworm, Diabrotica virgifera virgifera, being a major pest of maize and having populations showing increasing levels of resistance to hybrids expressing Bt pesticidal proteins, the cell mechanisms leading to mortality are not fully understood.

27p vitzuyu2711 29-09-2021 10 1   Download

The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants.

9p vijichea2711 28-05-2021 16 1   Download

A number of novel JA derivatives have been synthesized, isolated, and characterized. A clearer picture of jasmonate biosynthesis and functions has emerged that in many respects resembles the synthesis and functions of prostaglandins and leukotrienes in mammalians. Jasmonates are produced from a-linolenic acid from lipids of damaged plant membranes. The biosynthesis of these signalling compounds can be triggered through the activation of cell membrane receptors by pathogens, peptide signals, or wounding.

8p cothumenhmong7 09-09-2020 20 1   Download

Insect attack-wound in plant cells stimulate huge number of signal molecules to induce and activate defense system. Among these one of the intracellular signal molecules are small peptide systemin (18AAs long) produced by proteolytic cleavage in wounded cells from prosystemin (200As) precursor protein. Systemin then bind to its SR-160 (Systemin Cell Surface Receptor) protein (160 kD) and activate phospholipase A2 (PLA-2) that releases linolenic acid, which is initial precursor for JA biosynthesis.

4p kethamoi7 26-08-2020 11 1   Download

Cordyceps is a genus of fungi from the Cordycipitaceae family that grows using host insects as the nutritional source. This genus comprises approximately 500 species worldwide. In the present study, culturing 16 species from 3 genuses of Chu-Soh, namely, Cordyceps, Ophiocordyceps, and Isaria, were successfully artificially cultured We then specifically investigated the antitumor effects of Ophiocordyceps pulvinata on human cancer cells. Secondary metabolites of O.

13p nguaconbaynhay6 23-06-2020 19 2   Download

To determine the titer of baculovirus stock accurately based on green fluorescent protein (GFP) reporter, a recombinant baculovirus encoding FLAG-tagged protein and GFP reporter as bicistronic messenger was constructed. The bicistronic messenger RNA was transcribed using polyhedrin promoter and translated in infected Sf21 insect cells. The GFP reporter gene was expressed incap independent manner utilizing internal ribosome entry site (IRES) which helped in titration of baculovirus using end point titration method.

9p quenchua6 15-06-2020 10 1   Download

Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus’ fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains.

12p viuchiha2711 21-04-2020 10 0   Download