Interactive biological

Studies on nanoparticle-protein interaction have also not been studied. This would give information on ratio influence on the structural alteration of EGCg in EGCg-AuNPs and its functionality in vivo. Herein, the optimisation of EGCg-AuNPs will be determined with a large pool of stoichiometric synthesises of gold and EGCg. These optimised EGCg-AuNPs would then be studied with bovine serum albumin (BSA) for protein interactions, binding and thermodynamics.

177p runthenight07 01-03-2023 11 3   Download

In this paper, we present a novel global protein-protein interaction network alignment algorithm, which is enhanced with an extended large neighborhood search heuristics. Evaluated on benchmark datasets of yeast, fly, human and worm, the proposed algorithm outperforms state-of-the-art algorithms. Furthermore, the complexity of ours is polynomial, thus being scalable to large biological networks in practice.

11p tamynhan4 06-09-2020 15 4   Download

The binding of Thermomyces lanuginosa lipase and its mutants [TLL(S146A), TLL(W89L), TLL(W117F, W221H, W260H)] to the mixed micelles of cis-parinaric acid/ sodium taurodeoxycholate at pH 5.0 led to the quenching of the intrinsic tryptophan fluorescence emission (300–380 nm) and to a simultaneous increase in the cis-parinaric acid fluorescence emission (380–500 nm). These findings were used to characterize the Thermomyces lanuginosa lipase/cisparinaric acid interactions occurring in the presence of sodium taurodeoxycholate....

9p system191 01-06-2013 50 5   Download

Visual arrestin is converted from a ÔbasalÕ state to an ÔactivatedÕ state by interaction with the phosphorylated C-terminus of photoactivated rhodopsin (R*), but the conformational changes in arrestin that lead to activation are unknown. Small-angle X-ray scattering (SAXS) was used to investigate the solution structure of arrestin and characterize changes attendant upon activation. Wild-type arrestin forms dimers with a dissociation constant of 60 lM.

9p system191 01-06-2013 53 3   Download

The design of b-glycosidases with planed substrate specificity for biotechnological application has received little attention. This is mostly a consequence of the lack of data on the molecular basis of the b-glycosidase specificity, namely data on the energy of the noncovalent interactions in the enzymetransition state complex. In an attempt to fill this gap, sitedirected mutagenesis and enzyme steady-state kinetic experiments with different substrates were conducted, using as model a digestive b-glycosidase (glycoside hydrolase family 1) from Spodoptera frugiperda (Lepidoptera) (Sfbgly50).

10p system191 01-06-2013 45 4   Download

School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK; 2Shifa College of Medicine, Islamabad, Pakistan.Curcumin is a compound derived from the spice, tumeric. It is a potent inhibitor of the SERCA Ca2þ pumps (all isoforms), inhibiting Ca2þ-dependent ATPase activity with IC50 values of between 7 and 15 mM . It also inhibits ATPdependent Ca2þ-uptake in a variety of microsomal membranes, although for cerebellar and platelet microsomes, a stimulation in Ca2þ uptake is observed at low curcumin concentrations (,10 mM )....

10p system191 01-06-2013 37 5   Download

The amyloid protein precursor (APP) was incorporated into liposomes or phospholipid monolayers. APP insertion into liposomes required neutral lipids, such as L-a-phosphatidylcholine, in the target membrane. It was prevented in vesicles containing L-a-phosphatidylserine. The insertion was enhanced in acidic solutions, suggesting that it is modulated by specific charge/charge interactions. Surfaceactive properties and behaviour of APP were characterized during insertion of the protein in monomolecular films of L-a-phosphatidylcholine, L-a-phosphatidylethanolamine or L-a-phosphatidylserine. ...

9p research12 01-06-2013 42 4   Download

A comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal binding site of human and bovine serum albumins (HSA and BSA, respectively) and short peptide analogues was performed using potentiometry and spectroscopic techniques. It was found that while qualitative aspects of interaction (spectra and structures of complexes, order of reactions) could be reproduced, the quantitative parameters (stability and rate constants) could not.

9p research12 01-06-2013 38 4   Download

In the O2– generating flavocytochrome b, the membranebound component of the neutrophil NADPH oxidase, electrons are transported from NADPH to O2 in the following sequence: NADPH fi FAD fi heme b fi O2. Although p-iodonitrotetrazolium (INT) has frequently been used as a probe of the diaphorase activity of the neutrophil flavocytochrome b, the propensity of its radical to interact reversibly with O2 led us to question its specificity. This study was undertaken to reexamine the interaction of INT with the redox components of the neutrophil flavocytochrome b. ...

10p research12 01-06-2013 42 3   Download

Hevamine is a chitinase from the rubber tree Hevea brasiliensis. Its active site contains Asp125, Glu127, and Tyr183, which interact with the )1 sugar residue of the substrate. To investigate their role in catalysis, we have successfully expressed wild-type enzyme and mutants of these residues as inclusion bodies in Escherichia coli. After refolding and purification they were characterized by both structural and enzyme kinetic studies. Mutation of Tyr183 to phenylalanine produced an enzyme with a lower kcat and a slightly higher Km than the wild-type enzyme.

9p research12 01-06-2013 49 4   Download

The interaction between the c11ring and thececomplex, forming the rotor of theIlyobacter tartaricus ATP synthase, was probed by surface plasmon resonance spectroscopy andin vitro reconstitution analysis. The results pro-vide, for the first time, a direct and quantitative assessment of the stability of the rotor. The data indicated very tight binding between the c11ring and the cecomplex, with an apparent Kd value of approximately 7.4 nm.

13p research12 29-04-2013 47 3   Download

The serpin plasminogen activator inhibitor type 1 (PAI-1) plays an important role in physiological processes such as thrombolysis and ®brinolysis, as well as pathophysiological processes such as thrombosis, tumor invasion and metas-tasis. In addition to inhibiting serine proteases, mainly tissue-type (tPA) and urokinase-type (uPA) plasminogen activators, PAI-1 interacts with di€erent components of the extracellular matrix, i.e. ®brin, heparin (Hep) and vitronectin (Vn).

9p research12 29-04-2013 26 3   Download

ThemaltoseATP-binding cassette transporter ofSalmonella typhimurium is composed of a membrane-associated com-plex (MalFGK2) and a periplasmic receptor (MalE). In addition to its role in transport, the complex acts as a repressor ofmaltose-regulatedgene expressionand is subject to inhibition in the process of inducer exclusion. These activities are thought to be mediated by interactions of the ATPase subunit, MalK, with the transcriptional activator, MalT, and nonphosphorylated enzyme IIA of the glucose phosphotransferase system, respectively. ...

12p research12 23-04-2013 35 3   Download

The interactionofmany lytic cationic antimicrobial peptides with their target cells involves electrostatic interactions, hydrophobic effects, and the formation of amphipathic sec-ondary structures, such asahelices orbsheets. We have shown in previous studies that incorporating
30% D-aminoacids intoashortahelical lyticpeptidecomposedof leucine and lysine preserved the antimicrobial activity of the parent peptide, while the hemolytic activity was abolished.

12p research12 23-04-2013 35 2   Download

NBR1(named as next toBRCA1) was originally cloned as a candidate gene for the ovarian cancer antigen CA125, using expression cloning with the anti-CA125 Ig, OC125.NBR1 has been of interest due to its position close toBRCA1, although no involvement in breast or ovarian cancer has been demonstrated. Recently, the antigen CA125 has been cloned, and identi®ed as a new mucin, MUC16, entirely di€erent from NBR1.

8p research12 23-04-2013 45 3   Download

The interaction of bacterial endotoxins [lipopolysaccharide (LPS) and the
endotoxic principle lipid A], with high-den-sity lipoprotein (HDL) from serum was investigated with a variety of physical techniques and biological assays. HDL exhibited an increase in the gel to liquid crystalline phase transition temperature Tc and a rigidification of the acyl chains of the endotoxins as measured by Fourier-transform infrared spectroscopy and differential scanningcalorimetry.

10p tumor12 22-04-2013 38 3   Download

Upon mutation of Asp153 by asparagine, the catalytic activity of agmatinase (agmatine ureohydrolase, EC 3.5.3.11)fromEscherichia coliwas reduced to about 5% of wild-type activity. Tryptophan emission fluorescence (kmax ¼340 nm), and CD spectra were nearly identical for wild-type and D153N agmatinases. TheKmvalue for agmatine (1.6 ± 0.1 mM),aswellastheKi for putrescine inhibition (12 ± 2 mM)and the interaction of the enzyme with the requiredmetal ion,werealsonot alteredbymutation.

5p tumor12 22-04-2013 22 3   Download

Tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) has been known to induce tumor-specific apoptosis and to share the structural and functional char-acteristics with the proteins of TNF family. Recently, the crystal structure of humanTRAIL showed that TRAIL is a homotrimeric protein whose subunits contain mainly b-sheets. We characterized the structural changes of recombinant human TRAIL induced byacidification and the biological implication of the structural characteristics at acidic pH in the interaction with the lipid bilayer. ...

8p tumor12 22-04-2013 49 2   Download

Peptides corresponding to the N-terminus of skeletal myosin light chain 1 (rsMLC1 1–37) and the short loop of human cardiacb-myosin (hcM398–414) have been shown to interact with skeletal F-actin by NMR and fluorescence measurements. Skeletal tropomyosin strengthens the binding of the myosin peptides to actin but does not interact with the peptides. The binding of peptides cor-responding to the inhibitory region of cardiac troponin I (e.g. hcTnI128–153) to F-actin to form a 1 : 1 molar complex is also strengthened in the presence of tropomyo-sin. ...

13p tumor12 22-04-2013 37 2   Download

The calcium-sensing receptor (CaR) belongs to family C of the G-protein coupled receptor superfamily. The receptor is believed to exist as a homodimer due to covalent and non-covalent interactions between the two amino terminal domains (ATDs). It is well established that agonist binding to family C receptors takes place at the ATD and that this causes theATDdimer to twist.However, very little isknown about the translationof theATDdimer twist intoG-protein coupling to the 7 transmembrane moieties (7TMs) of these receptor dimers....

12p tumor12 22-04-2013 40 2   Download