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Internal ribosome entry site
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Internal ribosome entry sites (IRES) are segments of mRNA found in untranslated regions that can recruit the ribosome and initiate translation independently of the 5′ cap-dependent translation initiation mechanism. IRES usually function when 5′ cap-dependent translation initiation has been blocked or repressed.
15p
vicolorado2711
23-10-2020
12
0
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To determine the titer of baculovirus stock accurately based on green fluorescent protein (GFP) reporter, a recombinant baculovirus encoding FLAG-tagged protein and GFP reporter as bicistronic messenger was constructed. The bicistronic messenger RNA was transcribed using polyhedrin promoter and translated in infected Sf21 insect cells. The GFP reporter gene was expressed incap independent manner utilizing internal ribosome entry site (IRES) which helped in titration of baculovirus using end point titration method.
9p
quenchua6
15-06-2020
10
1
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Triple helical collagens are the most abundant structural protein in vertebrates and are widely used as biomaterials for a variety of applications including drug delivery and cellular and tissue engineering.
17p
viuchiha2711
21-04-2020
10
0
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Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity.
15p
viuchiha2711
21-04-2020
6
1
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The long uORF-burdened 5¢UTRs of many genes encoding regulatory proteins involved in cell growth and differentiation contain internal ribosomal entry site (IRES) elements. In a previous study we showed that utilization of the weak IRES of platelet-derived growth factor (PDGF2) is activated during megakaryocytic differentiation.
10p
system191
01-06-2013
33
3
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The leader proteinase (L pro ) of foot-and-mouth disease virus (FMDV) ini-tially cleaves itself from the polyprotein. Subsequently, L pro cleaves the host proteins eukaryotic initiation factor (eIF) 4GI and 4GII. This prevents protein synthesis from capped cellular mRNAs; the viral RNA is still trans-lated, initiating from an internal ribosome entry site. L pro cleaves eIF4GI between residues G674 and R675. We showed previously, however, that L pro binds to residues 640–669 of eIF4GI.
10p
awards
06-04-2013
34
2
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Translation initiation of hepatitis C virus (HCV) occurs through an internal ribosome entry site (IRES) located at its 5¢-end. As a positive-stranded RNA virus, HCV uses its genome as a common template for translation and replication, but the coordination between these two pro-cesses remains poorly characterized.
19p
galaxyss3
07-03-2013
33
3
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The soluble guanylyl cyclases (sGC), the receptor for nitric oxide, are hete-rodimers consisting of ana- and b-subunit. This study aimed to investigate the translational mechanism of the sGC b2-subunit. Two mRNA species for sGC b2 were isolated from human kidney.
10p
galaxyss3
07-03-2013
38
2
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Polypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splic-ing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site-dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear.
11p
viettel02
20-02-2013
33
2
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Refractoriness of hepatitis C virus internal ribosome entry site to processing by Dicer in vivo
13p
panasonic01
12-12-2011
50
2
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