Isothermal titration calorimetry (ITC)

In procedure 1, micellisation study by isothermal titration calorimetry (ITC) showed that the increase in Gl + concentration reduces the cmc and the micellisation enthalpy. Increasing of the micellisation entropy was also observed, suggesting desolvation of micellar structures as a consequence of electrostatic attraction with Gl+ ions. In procedure 2, titration of Gl+ with SDS solution at 7.

12p langthannam 29-12-2021 5 0   Download

Substrate binding of a family GH19 chitinase from a moss species, Bryum coronatum(BcChi-A, 22 kDa), which is smaller than the 26 kDa family GH19 barley chitinase due to the lack of several loop regions (‘loop-less’), was investigated by oligosaccharide digestion, thermal unfolding experiments and isothermal titration calorimetry (ITC).

11p cosis54 09-12-2012 54 1   Download

A rigorous understanding of any biological system that undergoes some change requires quantification of its thermodynamic and kinetic properties. Combined with structural detail, these data can be assimilated to enable a picture of the mechanism of the change (for example, going from the free to bound state in a biomolecular interaction, or from the folded to the unfolded state of a biomolecule). Isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) provide the thermodynamic data required for developing this understanding.

276p banhkem0908 24-11-2012 65 4   Download