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Leaf and petiole explants
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The objective of the present investigation was to study the effect of hygromycin and cefotaxime on cultured tissues of lettuce and based on these results, an efficient in vitro selection system for the further transformation was developed.
12p
chauchaungayxua8
03-10-2020
10
2
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An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1 ) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1 . Rooting was initiated with full or half media supplemented with 0.
7p
cothumenhmong3
22-02-2020
14
1
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The present investigation was conducted to optimize an efficient callus induction protocol in Carica papaya (cv. Pusa Nanha). Two different types of explants were collected from field grown papaya plants under study viz., leaf and petiole. The explants were surface sterilized and excised into 1-2 cm diameter and inoculated on basal MS medium supplemented with various concentrations of NAA, Kinetin and TDZ. The cultures were kept in dark inside the culture room at 25 ± 2°C and humidity 60-65%.
9p
trinhthamhodang3
12-02-2020
24
1
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Thidiazuron (TDZ) is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss.
6p
vibasque27
27-03-2019
29
1
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The RAPD profile of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. This protocol could be successfully used for the mass multiplication and germplasm conservation of this medicinal plant.
11p
vi4mua
24-02-2019
15
1
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Callogenesis using vegetative (leaf, stem, petiole) and floral (petal, pistil, anther) explants of Rosa gallica and R. hybrida was investigated using different combinations of 2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine, and gibberellic acid on modified Murashige and Skoog medium.
10p
vi4mua
24-02-2019
15
1
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