Lepidopteran insect

Hemolin, a plasma protein from lepidopteran insects, is composed of four immunoglobulin domains. Its synthesis is induced by microbial challenge. We investigated the biological functions of hemolin inManduca sexta.It was found to bind to the surface of bacteria and yeast, and caused these micro-organisms to aggregate. Hemolin was demonstrated to bind to lipopolysaccharide (LPS) from Gram-negativebacteriaand to lipoteichoic acid fromGram-positive bacteria.

8p research12 23-04-2013 36 2   Download

N-tert-Butyl-N,N¢-dibenzoylhydrazine and its analogs are nonsteroidal ecdysone agonists that exhibit insect molting hormonal and larvicidal activities. The interaction mode of those ecdysone agonists with the heterodimer of the ecdy-sone receptor andultraspiracle has not been fully elucidated. We expressed the ecdysone receptor B1 and the ultraspiracle of the lepidopteran,Chilo suppressalis,using anin vitro transcription/translation system and confirmed, using gel-shift assays, that the proteins functionas ecdysone receptors. We also analyzed their ligand-binding affinity. ...

10p tumor12 20-04-2013 45 3   Download

Carboxypeptidases were purified fromguts of larvae of corn earworm (Helicoverpa armigera), a lepidopteran crop pest, by affinity chromatography on immobilized potato carb-oxypeptidase inhibitor, and characterized by N-terminal sequencing. A larval gut cDNA library was screened using probes based on these protein sequences. cDNA HaCA42 encoded a carboxypeptidase with sequence similarity to enzymesofclan MC [Barrett,A.J.,Rawlings,N. D.& Woessner, J. F. (1998)Handbook of Proteolytic Enzymes. Academic Press, London.], but with a novel predicted spe-cificity towards C-terminal acidic residues....

12p dell39 03-04-2013 43 3   Download

The digestive tract of lepidopteran insects is extremely alkaline. In the pres-ent work, molecular adaptation of amylolytic enzymes to this environment was investigated in the flour moth Ephestia kuehniella, an important stored-product pest. Three digestivea-amylases [Ephestia kuehniellaa-amy-lase isoenzymes 1–3 (EkAmy1–3)] with an alkaline pH optimum were puri-fied from larvae and biochemically characterized.

16p viettel02 22-02-2013 43 2   Download

The complexes formed by partially folded human and bovine a-lactalbumin with oleic acid (OA) have been reported to display selective apoptotic activity against tumor cells. These complexes were named human (HAMLET) or bovine (BAMLET) alpha-lactalbumin made lethal to tumor cells.

15p mobifone23 18-01-2013 47 6   Download