Báo cáo khoa học: Binding affinity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity
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N-tert-Butyl-N,N¢-dibenzoylhydrazine and its analogs are nonsteroidal ecdysone agonists that exhibit insect molting hormonal and larvicidal activities. The interaction mode of those ecdysone agonists with the heterodimer of the ecdy-sone receptor andultraspiracle has not been fully elucidated. We expressed the ecdysone receptor B1 and the ultraspiracle of the lepidopteran,Chilo suppressalis,using anin vitro transcription/translation system and confirmed, using gel-shift assays, that the proteins functionas ecdysone receptors. We also analyzed their ligand-binding affinity. ...
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