Lineage tracing

Colorectal cancer (CRC) ranks as the second-leading cause of cancer-related death worldwide with metastases being the main cause of cancer-related death. Here, we investigated the genomic and transcriptomic alterations in matching adjacent normal tissues, primary tumors, and metastatic tumors of CRC patients.

26p viellison 28-03-2024 5 2   Download

Human bone marrow stromal cells (BMSCs) are an easily accessible and expandable progenitor population with the capacity to generate neural cell types in addition to mesoderm. Lineage tracing studies in transgenic animals have indicated Nestin+BMSCs to be descended from the truncal neural crest.

12p vicwell 29-02-2024 5 1   Download

Part 1 book "Mouse genetics - Methods and protocols" includes content: Generation of conditional knockout mice, generating mouse lines for lineage tracing and knockout studies, generating chimeric mice from embryonic stem cells via vial coculturing or hypertonic microinjection, genetic inducible fate mapping in adult mice using tamoxifen dependent cre recombinases,.... and other contents

259p oursky06 17-10-2023 4 2   Download

The planarian Schmidtea mediterranea is a master regenerator with a large adult stem cell compartment. The lack of transgenic labeling techniques in this animal has hindered the study of lineage progression and has made understanding the mechanisms of tissue regeneration a challenge. However, recent advances in single-cell transcriptomics and analysis methods allow for the discovery of novel cell lineages as differentiation progresses from stem cell to terminally differentiated cell.

17p viaristotle 29-01-2022 9 0   Download

The pairing of CRISPR/Cas9-based gene editing with massively parallel single-cell readouts now enables large-scale lineage tracing. However, the rapid growth in complexity of data from these assays has outpaced our ability to accurately infer phylogenetic relationships.

27p viarchimedes 26-01-2022 11 0   Download

Tumors can evolve and adapt to therapeutic pressure by acquiring genetic and epigenetic alterations that may be transient or stable. A precise understanding of how such events contribute to intratumoral heterogeneity, dynamic subpopulations, and overall tumor fitness will require experimental approaches to prospectively label, track, and characterize resistant or otherwise adaptive populations at the single-cell level.

21p viarchimedes 26-01-2022 6 0   Download

The investigation of skeletal muscle development is of importance in stock farming and biomedicine. It is still ambiguous that whether animals are born with the full set of skeletal muscle fibers or if the number of myofibers continues to increase postnatally.

6p viwyoming2711 16-12-2020 8 1   Download

The tauGFP reporter fusion protein is produced nearly ubiquitously by the TgTP6.3 transgene in TP6.3 mice and its localisation to microtubules offers some advantages over soluble GFP as a lineage marker. However, TgTP6.3Tg/Tg homozygotes are not viable and TgTP6.3Tg/− hemizygotes are smaller than wild-type.

16p viwyoming2711 16-12-2020 10 1   Download

Advances in fluorescence labeling and imaging have made it possible to acquire in vivo records of complex biological processes. Analysis has lagged behind acquisition in part because of the difficulty and computational expense of accurate cell tracking.

8p vikentucky2711 26-11-2020 8 0   Download

Next generation sequencing (NGS) of amplified DNA is a powerful tool to describe genetic heterogeneity within cell populations that can both be used to investigate the clonal structure of cell populations and to perform genetic lineage tracing.

16p vioklahoma2711 19-11-2020 4 1   Download

Recent advances in bioimaging and automated analysis methods have enabled the large-scale systematic analysis of cellular dynamics during the embryonic development of Caenorhabditis elegans. Most of these analyses have focused on cell lineage tracing rather than cell shape dynamics.

11p viflorida2711 30-10-2020 17 2   Download

Differentiated cells that arise from stem cells in early development contain DNA methylation features that provide a memory trace of their fetal cell origin (FCO). The FCO signature was developed to estimate the proportion of cells in a mixture of cell types that are of fetal origin and are reminiscent of embryonic stem cell lineage.

12p vikuala271 13-06-2020 12 0   Download

The Zane family was a remarkable one in early days, and most of its members are historical characters. The first Zane of whom any trace can be found was a Dane of aristocratic lineage, who was exiled from his country and came to America with William Penn. He was prominent for several years in the new settlement founded by Penn, and Zane street, Philadelphia, bears his name. Being a proud and arrogant man, he soon became obnoxious to his Quaker brethren. He therefore cut loose from them and emigrated to Virginia, settling on the Potomac river, in what was then known as Berkeley county.

159p believe11 20-02-2013 34 3   Download

The main ideas in this book trace their intellectual lineage to Benjamin Graham, whom I never knew but must thank posthumously, and Warren Buffett, whom I have the great fortune to know and from whose writings, talks, and conversations I have gained knowledge and insight. Neither of these men, of course, has any responsibility for this book’s content and no doubt would disagree with some of what it says, though it is written as a narrative interpretation of principles they developed, to which it tries to be faithful....

285p 951847623 09-04-2012 89 28   Download