Liquid chromatography mass spectrometry analysis
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Ebook "Peptidomics: Methods and strategies" describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types.
423p lucchinguyen 28-12-2023 7 2 Download
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A sensitive and specific liquid chromatography-tandem mass spectrometry (LCMS/MS) method was developed for the simultaneous analysis of 4 compounds used for treating hypertension as adulterants in herbal products. The method was validated in terms of selectivity, linearity, accuracy, limit of detection (LOD), limit of quantitation (LOQ), and precision in accordance with AOAC and ICH guidelines.
11p viwolverine 11-07-2023 11 5 Download
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Adirectinjection, multi residue analytical method separated in two chromatographic runs was developed utilizing scheduled analysis to simultaneously quantify 154 compounds, 84 precursors and 70 transformation products (TPs)/metabolites.
10p viginny 23-12-2022 9 3 Download
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Fatty acyl-Coenzyme A species (acyl-CoAs) are key biomarkers in studies focusing on cellular energy metabolism. Existing analytical approaches are unable to simultaneously detect the full range of short- , medium-, and long-chain acyl-CoAs, while chromatographic limitations encountered in the analysis of limited amounts of biological samples are an often overlooked problem.
12p viginny 23-12-2022 4 4 Download
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Insect repellents (IRs) are a group of organic chemicals whose function is to prevent the ability of insects of landing in a surface. These compounds have been found in the environment and may pose a risk to non-target organisms.
8p viginny 23-12-2022 9 3 Download
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A method for the trace analysis of polar micropollutants (MPs) by direct injection of surface water and groundwater was validated with ultrahigh-performance liquid chromatography using a core-shell biphenyl stationary phase coupled to time-of-flight high-resolution mass spectrometry.
9p viginny 23-12-2022 3 3 Download
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A peak-tracking algorithm was developed for use in comprehensive two-dimensional liquid chromatography coupled to mass spectrometry. Chromatographic peaks were tracked across two different chromatograms, utilizing the available spectral information, the statistical moments of the peaks and the relative retention times in both dimensions.
10p viginny 23-12-2022 5 3 Download
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Liquid Chromatography tandem mass spectrometry (LC-MS/MS) is the gold-standard approach for androgen analysis in biological fluids, superseding immunoassays in selectivity, particularly at low concentrations. While LC-MS/MS is established for analysis of testosterone and androstenedione, 5αdihydrotestosterone (DHT) presents greater analytical challenges.
12p viginny 23-12-2022 6 3 Download
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The aim of this work was to expand the applicability range of UHPSFC to series of synthetic and commercialized peptides. Initially, a screening of different column chemistries available for UHPSFC analysis was performed, in combination with additives of either basic or acidic nature.
12p viginny 23-12-2022 8 2 Download
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Due to the increasing number of therapeutic monoclonal antibodies (mAbs) used in the clinic, there is an increasing need for robust analytical methods to quantify total mAb concentrations in human plasma for clinical studies and therapeutic drug monitoring. We developed an easy, rapid, and robust sample preparation method for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.
10p viginny 23-12-2022 8 3 Download
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We developed a new multiplexed reversed phase liquid chromatography-high resolution tandem mass spectrometric (LC-MS/MS) method. The method is based on isobaric labeling with a tandem mass tag (TMT10-plex) and stable isotope-labeled internal standards, and was used to analyze amino acids in mouse brain microdialysis samples.
10p viginny 23-12-2022 5 3 Download
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We performed a systematic study of thirteen alkylamines used as ion-pairing reagents for ion-pair reversed-phase liquid chromatography (IP RP LC) separations of oligonucleotides on a C18 column. We proposed a method to classify the hydrophobicity of alkylamines by their retention in RP LC.
12p viginny 23-12-2022 5 3 Download
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Structural elucidation of compounds detected with liquid chromatography coupled to high resolution mass spectrometry is a challenging and time-consuming step in the workflow of non-targeted analysis and often requires manual validation of the results. Retention time, alongside exact mass, isotope pattern, fragmentation spectra, and collision cross-section, is valuable information for ruling out unlikely structures and increasing the confidence in others.
12p viginny 23-12-2022 6 2 Download
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An automated procedure for the simultaneous determination of six anionic pesticides, including glyphosate (GLY) and its transformation product aminomethylphosphonic acid (AMPA), was developed and applied to the analysis of environmental water samples.
9p vironald 15-12-2022 10 2 Download
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The aim of this study was to evaluate the potential of ultra-high performance supercritical fluid chromatography (UHPSFC) for peptide analysis by comparing its analytical performance to several chromatographic approaches based on reversed-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC) and mixed-mode liquid chromatography.
11p vironald 15-12-2022 6 2 Download
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In this paper, this drawback was intended to be addressed by the use of hexanol-based supramolecular solvents (SUPRAS) with restrictedaccess properties. The aim was to develop a fast and interference-free sample treatment workflow for the determination of opioids, cocaine, amphetamines and their metabolites in human hair.
11p vironald 15-12-2022 5 2 Download
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The paper samples were extracted with ethanol/water (1 : 1, v/v) mixture at 70°C for 2 h by using an incubator shaker. The extracts were passed through 0.2 μm filters before LC-MS/MS analysis. The method detection limit and method quantification limit of both PFOA and PFOS were 0.1 and 0.3 ng/g, respectively.
9p viporsche 03-11-2022 10 4 Download
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A determination method of ethylene glycol in animal feed by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was developed. The analysis was performed with a C18 column (150 mm × 4.6 mm, 3.5 µm), and a gradient mobile phase of 0.1 % formic acid solution and acetonitrile.
7p viedison 13-04-2022 11 1 Download
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The development of products that fully meet the criteria for a healthy diet is one of the main trends in nutrition quality improvement worldwide. Amaranth, a leader in protein quality, has significant potential. A comparative study of the protein complex in leaf biomass has been conducted for the first time for Amaranthus spp. grown for various uses. The aim of the study was to analyze the characteristics of the amino acid composition and identify the relationships among its components in the amaranth leaf biomass.
11p hanthienngao 30-11-2021 17 1 Download
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In the present study, an integrated in solution- in gel trypsin digestion approach, along with Isobaric tag for relative and absolute quantitation (iTRAQ)–Liquid chromatography/Mass spectrometry (LC/MS/MS) and data mining were performed to identify the proteomic profile and differentially expressed proteins in Anopheles culicifacies susceptible species A and refractory species B.
16p vibeauty 23-10-2021 6 1 Download