LJ Medium and MGIT

This study is aimed at providing early and accurate detection of NTM from isolated mycobacterium by using routine biochemical tests. In the laboratory, specimen were processed by modified Petroff”s and cultured in a pair of Lowenstein Jensen (LJ) medium and MGIT culture, samples were processed by NALC - NaOH method, and inoculated in MGIT culture tube. The LJ medium were incubated at 37°C for 8 weeks and LJ slopes were examined daily for one weak and then every week for colonies of acid fast bacilli (AFB). Once the growth appeared, it was confirmed by Ziehl-Neelsen staining.

10p gaocaolon5 14-06-2020 9 1   Download

Bacteriological confirmation plays a key role in the diagnosis of tuberculosis. Many systems have been developed in the recent years apart from traditional microscopy and culture on Lowenstein Jensen (LJ) medium. The objective of this study is to compare the rate of detection of Mycobacteria by acid fast staining, LJ medium and Mycobacteria Growth Indicator Tube (MGIT). Hundred patients with clinical suspicion of pulmonary tuberculosis attending a tertiary care hospital over a period of one and a half years were enrolled in the study.

8p caygaocaolon4 01-04-2020 8 1   Download