Magnaporthe grisea isolates

The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) causes blast disease on a broad range of plants including rice, foxtail millet and other species of the family Poaceae. The infected leaves of foxtail millet with typical spindle shaped lesions of light tan in colour, dusty gray appearance with necrotic borders were collected from various regions of Tamil Nadu during 2017 and 2018 and the pathogen was isolated from the samples. The collected isolates were named as TNFxM1 to TNFxM32.

6p cothumenhmong11 11-05-2021 14 2   Download

The pathogen is adaptable to adverse environmental conditions of widely fluctuating temperatures and relative humidity. It appears in irrigated low land or rain fed upland rice as well as in submerged or deep water rice. Rice was important crop in both Andhra Pradesh and Telangana states and losses due disease was also more. Keeping this in view we are conducting survey in both the states to identify the severity of disease.

10p trinhthamhodang7 31-08-2020 14 1   Download

Incubation period as a measure of infection efficiency for isolates of leaf blast pathogen in pearl millet and rice is an important parameter to testify host specificity as well as resistance against the pathogen. Incubation period (IP50) for leaf blast symptom development on pearl millet and rice using respective isolates (pearl millet isolate on pearl millet and rice isolate on rice) were estimated to be 4.98 and 6.23 days respectively at 26±1°C.

8p angicungduoc5 12-06-2020 7 2   Download

Blast (Magnaporthe grisea) is an economically important disease of finger millet in India. The cross infectivity of M. grisea isolates of leaf, neck and finger blast from finger millet can infect weed host viz., Pennisetum cenchroides, Pennisetum pureum and Cynodon dactylon but not the rice, foxtail millet and little millet. The vice versa pattern of cross infection were observed between the leaf, neck and finger blast pathogens.

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Blast is one of the most destructive and wide spread disease of rice. It is caused by Magnaporthe grisea (Herbert) Barr in different stages of its growth period. Of the 16 number of isolates collected from different locations of Assam, 6 physiologic races viz., IB8, IB33, IC17, ID1, ID10 and IF1 are found prevailing in this region. The isolate belongs to international race groups IB has not been detected so far in India. Under the race group IB, two new races were identified IB8 and IB33. Besides, in international race group ID and IF new race ID 10 and IF 1 respectively were also identified.

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A total of 24 blast infected samples were collected from major finger millet growing areas of Tamil Nadu and also from All India Coordinated Small Millets Improvement Project Centres (ICAR) in India. Pathogenicity test with Magnaporthe grisea isolates showed highly significant differences in virulence on the variety KM 252 in the severity of leaf blast. Among the five different media tested, the Oat Meal Agar medium (OMA) was found to be best for the growth of M. grisea isolates and all the isolates produced white colony colour. The mean mycelial growth was maximum in OMA medium with 80.

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A study was undertaken to isolate, identify and characterize the rice blast pathogen M. grisea from different rice growing regions. In the present study, ten isolates of M. grisea were categorized into different groups based on colony colour and texture. PCR was performed to identify the M. grisea isolates using the universal primers of 18S (ITS 1) and 28S (ITS 4). The PCR reaction allowed amplifying the fungal ITS fragments of 550 bp. All the isolates had the expected specific size of 550 bp which depicts molecular based confirmation of M. grisea.

9p chauchaungayxua3 07-02-2020 12 1   Download